聚(甲基丙烯酸-二乙烯基苯-三烯丙基异三聚氰酸酯)整体柱应用于质粒分析  

Application of Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) Monolith in Plasmid Analysis

作　　者：刘孟儒[1] 刘琳琳[2] 张琪[1] 崔伟佳[1] 张敏莲[1] 

机构地区：[1]清华大学化学工程系,北京100084 [2]北京理工大学化工与环境学院,北京100081

出　　处：《过程工程学报》2013年第2期287-291,共5页The Chinese Journal of Process Engineering

摘　　要：针对质粒的结构特点,合成了含大量10～300m超大孔的阴离子型聚(甲基丙烯酸二乙烯基苯三烯丙基异三聚氰酸酯)(PGDT)整体柱,经二乙胺修饰的PGDT具有密度为1.58mmol/g的阴离子交换功能基团.将其用于质粒标准品和细胞裂解液的分析,并与市售的无孔微球型色谱柱TSK DNA-NPR和整体柱型色谱柱CIM DEAE Disk Monolithic Column比较.PGDT整体柱分析质粒标准品时能有效将质粒与其他杂质分离,效果与市售TSK和CIM色谱柱相当;分析细胞裂解液时,PGDT整体柱和CIM色谱柱能将RNA、非核酸杂质分离,TSK则仅能将质粒和非质粒分离.合成的PGDT整体柱对质粒样品有良好的分析能力.Anion-exchange poly（glycidyl methacrylate-divinylbenzene-triallylisocyanurate） （PGDT） monolith with superporous pores with the diameters range of 10-300μm was synthesized, which was used to analyze plasmid standard and cell lysate. The analysis results were compared with commercial columns, including TSK DNA-NPR and CIM DEAE Disk Monolithic Column. The prepared PGDT monolith could effectively absorb plasmid and separate it from impurities, with its results similar to TSK and CIM.

关 键 词：聚(甲基丙烯酸二乙烯基苯三烯丙基异三聚氰酸酯) 整体柱 质粒DNA 分析 

分 类 号：O652.63[理学—分析化学] O657.7[理学—化学]