鲤鱼白细胞介素10基因组DNA克隆及序列分析  

Cloning and Sequence Analysis of Interleukin 10 Genomic DNA in Common Carp(Cyprinus carpio L.)

作　　者：冯祥汝[1] 陈义龙[1] 孙真[1] 贾生美[1] 王文东[2] 张俊辉[1] 杨振国[1] 卢强[1] 

机构地区：[1]吉林大学人兽共患病研究所,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国畜牧兽医》2013年第4期65-68,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30972277);吉林大学基本科研业务费项目(200903250)

摘　　要：为获得鲤鱼白细胞介素10(interleukin 10,IL-10)全长基因组序列,本研究利用鲤鱼IL-10全长cDNA序列(Gen-Bank登录号:JX524550),通过在两端非编码区设计引物,以提取的鲤鱼脾脏基因组为模板,使用PCR方法成功获得鲤鱼IL-10全长基因组序列。鲤鱼IL-10基因组全长2176bp,GenBank登录号为JX524551。将所获得的序列与其他物种IL-10基因组序列相比,结果发现都含有5个外显子,4个内含子,外显子在进化上相对保守,剪切位点都符合"gt......ag"规则;序列包含540bp的开放阅读框,编码179个氨基酸,有2段典型的IL-10氨基酸信号基序。The purpose of this study was to obtain the full-length sequence of common carp interleukin 10（IL-10） genomic DNA. The sequence of common carp IL-10 full-length cDNA（GenBank：JX 524550） was used. In this study, we designed two pairs of primers in its untranslated regions. Using the carp spleen genomic DNA as the template, we carried out PCR and obtained the IL-10 full-length genomic DNA sequence. The sequence was 2176 bp in length, and the GenBank accession number was JX524551. We compared the gained sequence with the other IL-10 and found their similarity of all harboring 5 exons and 4 introns. Moreover, all exons were comparatively conserved. The exons and introns splice sites were in accordance with ＂gt......ag＂ rules. Our results indicated that it was the sizes of introns that were crucial in the process of evolution.

关 键 词：鲤鱼 白细胞介素10 基因组 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]