无标记增强型检测Hg^(2+)的荧光DNA传感器  

A label-free enhanced fluorescence DNA biosensor for Hg^(2+) detection

作　　者：刘萍[1] 邓承雨[1,2] 金振国[1] 陈凤英[1] 庄肃凯[1] 

机构地区：[1]商洛学院化学与化学工程系,商洛726000 [2]江西师范大学化学化工学院,南昌330022

出　　处：《分析试验室》2013年第5期61-64,共4页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅自然科学基金项目(12JK0632);商洛学院基金项目(11SKY011)资助

摘　　要：基于Hg2+与T–T错配碱基对能特异性结合形成T-Hg2+-T结构以及结晶紫(CV)与单、双链DNA作用后荧光信号的差异,构建了一种荧光增强型检测Hg2+的DNA生物传感器。实验考察了不同序列DNA及溶液的pH、DNA与CV浓度比、稳定时间等因素对灵敏度的影响。在优化的条件下体系荧光效率和Hg2+浓度在2～40 nmol/L范围内呈良好的线性关系,检出限为0.7 nmol/L。并且高浓度的Ca2+,Mg2+等常见金属离子对Hg2+的检测没有干扰。方法为重金属Hg2+的检测提供了一个较好的荧光分析方法。A label-free signal-on＇fluorescence biosensor for detection of mercury （ Ⅱ ） in an aqueous solution is reported. This biosensor is based on the specific interaction of Hg2＋ with T-T mismatched base pairs and the fluorescence signal differences between ssDNA and dsDNA with crystal violet （CV）. The influence factors on detection sensitivity, such as different sequences of DNA, pH, DNA/CV concentration ratio, stability time and so on, have been discussed. Under the optimum experimental conditions, the system fluorescence intensity gave a lanear response to the concentration of Hg2＋ in a range from 2 to 40 nmol/L. The detection limit for Hg2＋ was 0. 7 nmol/L. We also found that even at high concentration, the common metal cations such as Ca2＋ , Mg2＋ in the system had no impact on the detection of Hg2＋. It can provide a good fluorescence analysis method for the detection of Hg2＋ in the heavy metal polluted environment.

关 键 词：结晶紫 Hg2+ 荧光DNA生物传感器 T-Hg2+-T 

分 类 号：O657.3[理学—分析化学]