基于氯冉酸荷移分光光度法测定乙酰螺旋霉素含量  

Spectrophotometric determination of acetylspiramycin based on its charge transfer reaction with chloranilic acid

作　　者：庞秋霞[1] 孙雪花[2] 柴红梅[2] 高楼军[2] 

机构地区：[1]延安大学医学院,延安716000 [2]延安大学化学与化工学院,延安716000

出　　处：《分析试验室》2013年第5期81-83,共3页Chinese Journal of Analysis Laboratory

基　　金：陕西省重点实验室项目(12JS119);延安大学自然科学专项基金重点项目(YDZD2011-06)资助

摘　　要：研究了乙酰螺旋霉素和氯冉酸在甲醇介质中发生的荷移络合反应,确定了最佳条件,从而建立了一种简便快速测定乙酰螺旋霉素的荷移分光光度法。荷移络合物的最大吸收波长为λ=531 nm,络合比为1∶1,该络合物的吸光度与乙酰螺旋霉素的浓度在1.2～20 mg/L范围内呈线性关系,线性相关系数r=0.9998,检出限为0.5 mg/L,相对标准偏差为1.2%,回收率为99.5%～101.2%。本方法用于对乙酰螺旋霉素片含量的测定,结果较为满意。In a methanol medium, the charge transfer reaction between acetylspiramycin and chloranilic acid was investigated by spectrophotometry. The experimental optimum conditions were established, and a simple and rapid method for the determination of acetylspiramycin was developed. The maximum absorption wavelength of the charge transfer complex was 531 nm, and the complex ratio was 1： 1. The concentration of acetylspiramycin obeyed Beer＇s law in the range of 1.2 ~ 20 mg/L, and the linear correlation coefficient was r = 0. 9998. The detection limit was 0.5 mg/L, the relative standard deviation was 1.2%, and the recoveries were 99.5% - 101.2%. This method has higher sensitivity, good selective and high stability. The method was applied to the determination of acetylspiratamycin in tablets with satisfactory results.

关 键 词：分光光度法 荷移反应 乙酰螺旋霉素 氯冉酸 

分 类 号：O657.3[理学—分析化学]