益胃汤对子宫内膜异位症大鼠异位内膜VEGF和MVD表达的影响  

Study on Effect of YWT on VEGF and MVD Expressions of Ectopic Endometrium in EMs Rat Model

作　　者：罗晓红[1] 郑燕[1] 闫新林[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《辽宁中医杂志》2013年第4期803-805,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：四川省科技厅支撑项目(2010SZ0196)

摘　　要：目的:观察益胃汤(YWT)对子宫内膜异位症(EMs)血管生成的抑制作用和机制。方法:采用大鼠自体子宫内膜移植建立子宫内膜异位症(内异症)大鼠模型。将造模成功大鼠随机分为模型对照组、达那唑对照组、反应停对照组、益胃汤高、中、低剂量组,另设假手术对照组,连续给药4周后,采用免疫组化的方法检测异位和在位子宫内膜MVD和VEGF的表达。结果:YWT治疗4周后,与模型对照组比较,YWT高、中剂量组异位子宫内膜的MVD和VEGF均较模型对照组明显低表达(P<0.05),阳染细胞数较模型对照组明显减少,染色深度减轻。结论:YWT具有较好的抗EMs血管生成作用,其作用机制与抑制血管生成因子VEGF的水平有关,该作用可能是通过免疫调节实现的。Objective ： To observe the mechanism of YWT （Yiweitang） in inhibiting EMs （ Endometriosis ） angiogenesis. Methods ： The EMs rat model was established by autologous endometrial transplantation. The EMs rat models were randomly divided into 6 groups ： model control group, Thalidomide control group, Danazol control group, YWT high dose group ,YWT middle dose group, YWT low dose group and sham - operation control group. The VEGF and MVD expressions of both ectopic endorhetrium and normal endometrium were tested by immunohistochemical method after four weeks continuous administration. Results ： After four weeks continuous administration of YWT, compared with model control group, the VEGF and MVD expressions of YWT high dose and middle dose groups were in low expression（ P 〈 0. 05 ）. Compared with the model control group, the positive stained cell number of YWT high dose and middle dose groups was decreased significantly, and the stain was also reduced Significantly. Conclusion：YWT has the function connected with the expressions of VEGF to inhibit EMs angiogenesis. The effect may be achieved through immunomociulatlon.

关 键 词：益胃汤 子宫内膜异位症 MVD VEGF 

分 类 号：R285.5[医药卫生—中药学]