低密度脂蛋白颗粒大小测定及意义  

Measurement of low density lipoprotein particle size and its significance

作　　者：赵凤丽[1] 王拥军[1] 周卫东[1] 陈保生 薛红 周茗[1] 杨静芳[1] 

机构地区：[1]首都医科大学宣武医院神经内科实验室,北京100053 [2]中国医学科学研究院基础医学研究所

出　　处：《临床检验杂志》2000年第2期70-72,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：初步建立低密度脂蛋白 (LDL)颗粒大小的测定方法并探讨其意义。采用 2 0～ 16 0g/L非变性聚丙烯酰胺梯度凝胶电泳 (12～ 16℃ ,12 5V ,电泳 2 4小时 )分离LDL亚组分并进行凝胶扫描测定LDL颗粒大小的分布。在有颈动脉粥样硬化的病例组和正常对照组之间LDL亚组分分布有差异 ,前者以小而致密的B型为主 ,而后者以大而疏松的A型为主。结果表明 ,测定LDL颗粒大小对动脉粥样硬化性疾病的预测有一定的意义。In this study non denaturing 20～160 g/L polyacrylamide gradient gel electrophoresis was used to separate LDL subfractions.After electrophoresis the gel was scaned and LDL particle size was obtained from the migration of standards of known diameter.In our result the small dense LDL subfraction is more prevalent in case group who developed carotid atherosclerosis than control.It is concluded that measurement of LDL particle size may have certain significance in predicting atherosclerosis.

关 键 词：低密度脂蛋白 颗粒大小 动脉粥样硬化 

分 类 号：R543.504[医药卫生—心血管疾病] R446.112[医药卫生—内科学]