云芝糖肽对乳腺癌患者PBMC中TLR信号通路MyD88途径的调节机制  

Immunomodulatory effects of polysaccharopeptide on TLR signaling pathway of PBMC through MyD88 in patients with breast cancer

作　　者：王静[1] 余爽[1] 陈楚[1] 董冰[1] 鲍依稀[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400010

出　　处：《中国免疫学杂志》2013年第3期236-239,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81274144);重庆市科委课题项目(CSCT 2009BB5399);重庆市教委课题项目(KJ110303);重庆市卫生局中医药科技项目(2010-2-147)资助

摘　　要：目的:探讨云芝糖肽(PSP)对乳腺癌患者外周血单个核细胞(PBMC)中TLR信号通路MyD88途径的免疫调节机制。方法:体外分离乳腺癌患者(20例)PBMC并将每份细胞分为2组,设为对照组和PSP组,采用实时荧光定量PCR(Q-PCR)检测各组PBMC中TLR信号通路MyD88途径相关基因的表达情况并进行定量分析,同时使用Western blot检测信号末端蛋白的表达情况。结果:PSP能够显著提高TLR信号通路中TLR4、TLR5、TLR6、IRAK4、TRAF6、IRF5、TAK1、IKKα、NF-κB、ERK、P38、JNK、AP-1、IL-1β和IL-8基因的相对表达量(P<0.01),信号末端蛋白NF-κB、AP-1和IRF5均有上调。结论:PSP对乳腺癌患者PBMC中TLR通路的调控可能主要是通过MyD88途径促使终端效应因子的释放从而发挥其免疫调节作用。Objective：To detect the effects of polysaccharopeptide （PSP） on TLR signaling pathway in breast cancer patient＇s peripheral blood mononuclear cells （PBMCs）. Methods： After isolation of PBMC from breast cancer patient, cells were divided into control group and PSP group. Quantitative real-time PCR （Q-PCR） and Western blot were used to detect the related gene and protein expression of TLR pathway regulated by PSP. Results： Genes in TLR/MyD88 pathway were up-regulated significantly by PSP： TLR4, TLRS, TLR6, IRAK4, TRAF6, IRFS, TAK1, IKKct, NF-KB, ERK, P38, JNK, AP-1, IL-113 and IL-8 （P 〈 0. O1 ）. Meanwhile, the terminal effect proteins （NF-RB, AP-1 and IRF5 ） were up-regulated significantly by PSP. Conclusion： PSP takes its immunoregu- latory effect on TLR signaling pathway through MyD88 in patients with breast cancer.

关 键 词：云芝糖肽 TLR 免疫调节 MyD88途径 

分 类 号：R446.61[医药卫生—诊断学]