植物检疫性有害生物蔗扁蛾的PCR快速鉴定  

Rapid PCR-based identification of the plant quarantine pest Opogona sacchari (Bojer)

作　　者：王星 徐淼锋[2] 廖力[2] 何明远[3] 刘双清 

机构地区：[1]植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128 [2]珠海出入境检验检疫局技术中心,广东珠海519015 [3]湖南省农业科学院植物保护研究所,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2013年第2期173-175,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30800710);国家质量监督检验检疫总局项目(2009IK277)

摘　　要：基于PCR分子检测技术,建立了进境植物检疫性有害生物蔗扁蛾(Opogona sacchari)快速、准确的鉴定方法。该方法对待检样品的基因组DNA经PCR扩增后进行凝胶电泳图分析,实现对待检样品的准确鉴定,检测时间缩短至8 h内,检测基因组DNA质量浓度限度为1 ng/μL,最适检测限为10 ng/μL以上。Based on polymerase chain reaction （PCR）, a rapid and accurate identification method was established for detection of the import plant quarantine pest Opogona sacchari. Genomic DNA of the samples was used as template in PCR amplification, and the PCR products were analyzed by gel electrophoresis. By PCR amplification, the detection time for Opogona sacchari was shortened to less than 8 hours. The test limit for genomic DNA concentration was 1 ng/μL, and the concentration above 10 ng/μL was the optimal.

关 键 词：检疫性有害生物 蔗扁蛾 聚合酶链式反应 

分 类 号：S851.34[农业科学—预防兽医学]