猪血清中抗猪丹毒丝菌特异性IgG的纯化  

Purification of Erysipelothrix rhusiopathiae specific IgG from swine serum

作　　者：胡云霏[1] 钱幸[1] 王淑琴[1] 余兴龙[1] 刘晓波[1] 罗璋[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2013年第2期183-187,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科学技术厅重点项目(2011FJ2017)

摘　　要：为了获得纯度高、特异性好的抗猪丹毒丝菌特异性IgG,采用抗原反向吸附结合重组葡萄球菌蛋白A(SPA)亲和层析法对猪丹毒丝菌阳性血清进行纯化。结果表明,血清用量对IgG的纯化结果影响不大,而细菌的用量则与IgG的获得量成正比;用猪丹毒丝菌与阳性血清吸附,初步纯化出的猪丹毒丝菌特异性IgG,再用SPA亲和层析法特异性的浓缩和纯化IgG,获得的抗体含量为240μg/mL,即从8 mL阳性血清中获得360μg猪丹毒丝菌特异性IgG,其纯度接近100%,可用于噬菌体展示的靶分子。To obtain Erysipelothrix rhusiopathiae（E, rhusiopathiae） special IgG with high specificity and purity, we use the antigen reverse adsorption combined with recombination protein A （SPA） of staphylococcus affinity chromatography to purify the specific IgG from swine serum. The result showed that the volume of serum showed little influence on the amount of IgG obtained, while the amount of bacteria was positively related to the yield of IgG E.rysipelothrix rhusiopathiae specific IgG was first purified from swine serum, and then subjected to SPA affinity chromatography, the final concentration of the IgG obtained is 240 μg/mL, thus, we get 360 μg antibody from the 8 mL swine serums, the purity of which is about 100% which can be applied in phage display.

关 键 词：猪丹毒丝毒 特异性免疫球蛋白G(IgG) 猪血清 IgG纯化 纯度 重组葡萄球菌蛋白A凝胶(SPA) SDS–聚丙烯酰胺凝胶电泳(SDS PAGE) 

分 类 号：S855[农业科学—临床兽医学]