蛇毒类凝血酶基因工程的研究进展  

Research Progress on Cloning of Snake Venom Thrombin-like Enzymes

作　　者：李家祺[1] 李谦[2] 唐松山[1] 李红枝[1] 

机构地区：[1]广东药学院基础学院广东省重点生物活性实验室,广东广州510006 [2]中国药科大学生命科学与技术学院生物化学教研室,江苏南京210009

出　　处：《药物生物技术》2013年第2期167-170,共4页Pharmaceutical Biotechnology

基　　金：中山市科技项目(No.2009H015)

摘　　要：蛇毒类凝血酶(Thrombin-like enzyme,TLE)是蛇毒中与血浆凝血酶性质相似的一类丝氨酸蛋白酶,所不同的是在蛇毒类凝血酶结构中已经没有纤维蛋白稳定因子激活组分。由于蛇毒类凝血酶在新药研究中扮演重要角色,比如来自Bothrops jararaca和Bothrops atrox蛇毒的立芷雪、来自东北白眉蝮蛇毒的邦停和来自尖吻蝮蛇毒的苏灵,它们是具有止血作用的新药;来自Gloydius shedaoensis和Gloydius ussuriensis蛇毒的东菱克栓酶、来自白眉蝮蛇和尖吻蝮蛇毒的降纤酶、来自Calloselasma rhodostoma蛇毒的安克洛酶和来自Crotalus adamanteus蛇毒的Crotalase,它们是具有溶栓作用的新药。这些药用蛋白质的来源和产量因有限的蛇毒原料而有很大限制,通过基因克隆可解决资源问题。文章综述了蛇毒类凝血酶的基因结构、糖基化特点和各种重组表达体系,为大量制备供临床和基础研究使用奠定基础。Snake venom thrombin-like enzymes （snTLE） are one kind of serine proteases similar to thrombin from blood, but the basic difference between them is that snTLE has no XIII-activated domain to lead thrombosis in vivo or in vitro. Because snake venoms play very important role in pharmaceutics ,for example Reptilase from Bothrops jararaca or Bothrops atrox snake venom,Bangtin from Agkistrodon halys snake venom, or Suling from Agkistrodon acutus snake venom, are hemostatic drugs. Defibrase from Gloydius shedaoensis, Gloydius ussuriensis ,Agkistroclon halys, or Agkistrodon acutus snake venom,Ancrod from Calloselasma rhodostoma snake venom, or Crotalase from Crotalus adamanteus snake venom,is thrombolytic drug. So the shortage of snake venom source can be over- come by using gene recombination method. The gene structures, protein glycosylation, and all kinds of cloning expression systems for snTLE protein are summarized in the paper, which will provide a basis for large-scale production of some snTLE proteins.

关 键 词：蛇毒类凝血酶 基因序列 糖基化 基因表达系统 

分 类 号：Q78[生物学—分子生物学]