水霉拮抗菌拮抗活性物质的分离鉴定  

Isolation and Identification of Antagonistic Compound From functional Strain of Antagonistic to Saprolegnia parasitica

作　　者：赵春晖[1] 杨桂文[1] 秦玉广[2] 彭少卿[1] 王红丽[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东省淡水水产研究所,山东济南250013

出　　处：《科技信息》2013年第7期1-2,7,共3页Science & Technology Information

基　　金：山东省科技发展计划项目研究成果;项目名称<基因工程技术生物(生态)防治水霉病技术研究>;项目编号2010GNC10929

摘　　要：本实验采用琼脂扩散法证实,功能拮抗菌株TCCC11322克隆及培养上清,对寄生水霉XJ001生长都有明显的抑制效果,在此实验基础上,对无菌培养上清,进行粗蛋白、粗多糖和肽类物质的分离提取,并对各组分进行了水霉生长拮抗活性检验鉴定,结果证明,培养上清中的分泌性蛋白成分对水霉生长具有明显的抑制效果。Using agar diffusion method in this experiment, It was found that both the clone and culture supernent from antagonistic strain TCCC11322 showed visible inhibition effect in the growth of Saprolegnia parasitiea strain XJ001.Using culture supernet,crud protein,crud polysaceharides and small peptide compounds was extracted respectively and further investigation was conducted.The experimental results demonstrate that the secret suprenent protein is the active antagonistic constituent to Saprolegnia parasitiea.

关 键 词：水霉病 生物拮抗 拮抗活性物质 生物防治 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]