一株橡胶树白粉病生防菌的鉴定及在橡胶树叶片定殖规律研究  

Identification of a bacterial isolate for biocontrol of the rubber tree powdery mildew and its colonization in rubber tree leaves

作　　者：黄有航[1] 谭志琼[1] 王寅寒[1] 张荣意[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室/海南大学环境与植物保护学院,海口570228

出　　处：《植物保护》2013年第2期77-81,共5页Plant Protection

基　　金：国家科技支撑计划(2007BAD48B04);海南大学热带作物种质资源保护与开发利用教育部重点实验室开放基金;海南大学"211工程"建设经费

摘　　要：从橡胶树根际土壤中分离到一株对橡胶树白粉病(Oidium heveae)有较强拮抗作用的细菌S43,对其进行形态、生理生化特征以及gyrB基因序列分析,将S43鉴定为枯草芽胞杆菌(Bacillus subtilis)。采用伤叶和不伤叶的方法,研究了标记菌株S43*在橡胶叶片中的定殖动态。结果表明:S43*能在橡胶树叶片上较好地定殖,接种后第1天菌量达到最大为1.34×105 cfu/g,随着时间推移标记菌株在叶片定殖数量呈总体下降趋势,接种15d后达到稳定,为1.9×102 cfu/g。The bacterial isolate S43 was obtained from rhizosphere soils of rubber tree plantation, with a strong antagonism to the powdery mildew Oidium heveae B. A, Steinm on rubber trees. The isolate S43 was identified as Bacillus subtilis based on its morphological features, physiological and biochemical characteristics and gyrB gene sequence. With and without injuring leaves, colonization dynamics of the marked strain S43 ＊ was investigated on rubber trees. The results showed that the marked strain S43 ＊ could colonize rubber tree leaves well. Within 1 day after inoculation, the population of the marked strain S43 ＊ reached the maximum, which was 1.34 × 105 cfu/g. As time went on, the population of the marked strain S43 ＊ decreased gradually. After 15 days, the inoculated population was stable, which was 1.9× 102 cfu/g.

关 键 词：橡胶树 白粉病 芽胞杆菌 鉴定 定殖 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]