逆转录环介导等温扩增技术检测南芥菜花叶病毒  

Detection of Arabis mosaic virus by the reverse transcription loop-mediated isothermal amplification method

作　　者：郭立新[1] 谭毅[1] 刘永丰[1] 邓丛良[2] 段维军[3] 许邹亮 

机构地区：[1]北仑出入境检验检疫局,宁波315800 [2]北京出入境检验检疫局,北京101312 [3]宁波出入境检验检疫局,宁波315012 [4]北京蓝谱生物科技有限公司,北京100086

出　　处：《植物保护》2013年第2期91-95,95,共5页Plant Protection

基　　金：宁波检验检疫局科研项目(甬K07-2011);国家质检总局课题(2011IK169;2012IK297;2011IK157);质检公益性行业科研专项(201110035);国家科技支撑计划(2012BAK11B02)

摘　　要：根据南芥菜花叶病毒外壳蛋白基因序列设计并合成了2组RT-LAMP引物,通过筛选试验,最终确定Ar4组引物为最佳引物,建立了南芥菜花叶病毒的RT-LAMP检测方法。同时,通过实时浊度仪和钙黄绿素分别对检测结果进行了判断。特异性和灵敏度试验结果显示,RT-LAMP方法快速、特异且灵敏,其灵敏度与普通RT-PCR法一致。Two sets of primers were designed and synthesized based on coat protein gene of Arabis mosaic virus （ArMV） for reverse transcription loop-mediated isothermal amplification （RT-LAMP） assay. Based on primer screening, the set Ar4 was selected as the optimal primers. A simple and sensitive RT-LAMP method was estab- lished to detect ArMV. Amplification products were detected by the real time turbidimeter and using calcein sepa- rately. Specificity and sensitivity assay showed that the RT-LAMP detection was rapid, specific and sensitive, and it was the same sensitive as RT-PCR.

关 键 词：南芥菜花叶病毒 逆转录环介导等温扩增 检测 

分 类 号：S432.41[农业科学—植物病理学]