响应面法优化透析制备低分子质量人参糖肽的工艺  

Optimized Dialysis Preparation of Low Molecular Mass Glycopeptide from Panax ginseng Using the Response Surface Methodology

作　　者：王颖[1] 罗浩铭[2] 陈英红[1] 姜瑞芝[1] 

机构地区：[1]吉林省中医药科学院,吉林长春130012 [2]吉林大学药学院,吉林长春130021

出　　处：《林产化学与工业》2013年第2期37-40,共4页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(81173532);吉林省科技发展计划项目(201201086)

摘　　要：应用响应面法对透析制备低分子质量人参糖肽的工艺进行研究。考察了超滤液透析前的配制质量浓度(A)、透析时间(B)以及透析外液用水量(C)3个因素对总糖肽含量的影响。得到的透析的优化工艺为:超滤液透析前的配制质量浓度0.6 g/mL,透析时间5h 1 min 48s,透析外液用水量3.92 L,实际测定值为79.26%,与模型预测值(78.99%)基本相符。采用透析法能够制备出纯度相对较高的人参糖肽,并通过响应面法得到了一个能较好预测实验结果的模拟方程。Response surface methodology （RSM） was used to study the technology of preparation of low molecular mass glycopeptide from panax ginseng by dialysis. The effects of three factors, i.e. , the ultrafihrate concentration before dialysis （A） , dialysis time （B） and water consumption （C） on the content of glycopeptide were investigated. The optimal conditions for dialysis were ultrafiltrate concentration 0.6 g/mL, dialysis time 5 h 1 min 48 s and water consumption 3.92 L. The experimental values （79.26 % ） agree with the predicted values（78.99 % ）. The panax ginseng peptide with relatively high purity can be prepared by dialysis, A model equation obtained can be used to predict the experiment through the RSM.

关 键 词：人参 糖肽 透析 响应面法 

分 类 号：TQ35[化学工程] O657.3[理学—分析化学]