Py-GC-MS研究水提取前后银杏叶化学成分的变化  

Component Varieties of Ginkgo biloba Leaves before and after Extraction with Water by Py-GC-MS

作　　者：陶冉[1,2,3,4] 王成章[1,2,3,4,5] 孔振武[1,2,3,4,5] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042 [5]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2013年第2期55-60,共6页Chemistry and Industry of Forest Products

基　　金：'十二五'国家科技支撑计划资助项目(2012BAD21B00);林业公益性行业专项(201004007);国家自然科学基金资助项目(30871986)

摘　　要：对水提前后的银杏叶样品在423℃进行四甲基氢氧化铵在线甲基化裂解,利用裂解-气质联用(Py-GC-MS)技术,结合Nist02质谱标准库,共指认了43种化学成分,并推断出相对应的原始化学结构,标明各自的GC含量,归属了其中主要化合物的化学结构类型。结果显示:12个碳以下的小分子化合物的总GC含量由水提前的38%变为水提后的30%;20个碳以下的长链脂肪酸(醇)类化合物的总GC含量由水提前的17%变为水提后的20%;银杏烷基(烯基)酚(酸)类、甾醇类及20个碳以上脂肪酸等成分的GC含量由水提前的45%变为水提后的50%。Ginkgo biloba leaves before and after extraction with water were treated by online pyrolytic methylation using tetrameth- flammonium hydroxide（TMAH） in the injector at 423 ~C. Forty-three compounds were identified and inferred by Py-GC-MS with the aid of Nist02 standard MS database. The GC content of original chemical compounds was determined by normalization method. The main compounds were classified. Comparing between Ginkgo biloba leaves before and after extraction with water, the results showed that the GC content of small molecule compounds with less than 12 carbon atoms reduced from 38% to 30% , and the GC content of aliphatie acid with less than 20 carbon atoms increased from 17% to 20%. The GC content of gingkolic acid, steroids and aliphatic acid compounds with more than 20 carbon atoms increased from 45% to 50%.

关 键 词：银杏 在线甲基化 裂解-气质联用 

分 类 号：TQ35[化学工程]