黄杞叶和片姜黄提取物及其组方对大鼠急性心肌缺血的保护和协同作用  

Synergistic and protective effects of extracts from Engelhardtia roxburghiana or Rhizoma Wenyujin Concisum on acute myocardial ischemia in Rats

作　　者：唐庆年[1] 伍倩[1] 田淑娟[2] 毛峻琴 

机构地区：[1]解放军181医院,广西桂林530000 [2]第二军医大学药学院,上海200433 [3]解放军85医院,上海200052

出　　处：《药学实践杂志》2013年第2期120-123,157,共5页Journal of Pharmaceutical Practice

摘　　要：目的研究黄杞叶和片姜黄的提取物联合应用防治大鼠急性心肌缺血的合理性。方法根据权重配方法设计6个不同配伍联合用药组,采用冠脉动脉结扎法制备大鼠急性心肌缺血模型,以心肌梗塞范围和血清肌酸激酶(CK)、乳酸脱氢酶(LDH)为指标,评价大鼠急性心肌缺血的损伤程度。采用非线性混合效应分析研究联合用药的相互作用。结果黄杞叶和片姜黄的提取物均能够保护心肌细胞,显著降低冠脉结扎造成急性心肌缺血大鼠血清中CK和LDH,缩小心肌梗死体积。非线性混合效应分析显示黄杞叶和片姜黄其提取物联用时有明显的协同作用。结论黄杞叶和片姜黄的提取物对大鼠急性心肌缺血具有显著的保护作用,两者合用具有明显的协同作用。Objective To study the optimal combined use of extracts from Engelhardtia roxburghiana with Rhizoma Wenyujin Concisum on acute myocardial ischemia in rats. Methods Six combined treatment groups were designed according to the weighted modification method, acute myocardial ischemia rats were induced by coronary artery ligation. The size of myocardial infarction and the levels of LDH and CK in serum were measured to evaluating acute myocardium ischemia injury. The nonlinear mixed effect model （ NONMEM ） was used to quantitatively assess the effects of combined use of drugs. Results Extracts from Engelhardtia roxburghiana or Rhizoma Wenyujin Concisum could protect myocardial cells, significantly reduce serum CK and LDH in acute myocardial ischemia rats. NONMEM analysis showed that there were obvious synergistic effects of the combination of Engelhardtia roxburghiana exctract with Rhizoma Wenyujin Concisum exctract on myocardial ischemia. Conclusion Extracts from Engelhardtia roxburghiana or from Rhizoma Wenyujin Concisum had synergistic and protective effects on acute myocardial ischemia in rats.

关 键 词：急性心肌缺血 黄杞叶 片姜黄 提取物 协同作用 

分 类 号：R965[医药卫生—药理学]