微量输液泵应用于逆行胰胆管注射牛黄胆酸钠大鼠重症急性胰腺炎造模的效果研究  

Microinfusion Pump Applied in Retrograde Biliopancreatic Duct Injection of Sodium Taurocholate in the Induction of Severe Acute Pancreatitis Model in Rats

作　　者：郑晓博[1,2] 陈光宇[2] 黎鹏武 蒋志伟[2] 邹洪[2] 文艺[2] 周超[3] 汤礼军[2] 

机构地区：[1]泸州医学院附属医院肝胆外科,四川泸州646000 [2]成都军区总医院全军普外中心,成都610083 [3]成都市第六人民医院肝胆外科,成都610051

出　　处：《西南军医》2013年第3期241-243,共3页Journal of Military Surgeon in Southwest China

基　　金：胰腺相关疾病四川省青年科技创新研究团队基金资助(四川省2011年第五批科技计划项目2011JTD0010)

摘　　要：目的探讨微量输液泵应用于逆行胰胆管注射牛黄胆酸钠大鼠重症急性胰腺炎造模的优势。方法 108只健康雄性wistar大鼠分为手推法组(A组)、微量输液泵法组(B组)和假手术组(C组),每组36只,其中18只大鼠用于观察24h生存率,其余18只大鼠分3、6、12h三个时间点,每时间点分配6只。A组采用手推法经肠壁穿刺逆行胰胆管注射5%牛黄胆酸钠造成重症急性胰腺炎模型,B组采用微量输液泵法,C组仅行开腹关腹手术;于处理后3、6、12h取血、胰腺组织,检测血清淀粉酶水平、胰腺组织病理组织学评分,并观察24h生存率。结果 A组和B组各时间段血清淀粉酶和胰腺病理组织学评分均显著高于C组,B组各时间段血清淀粉酶和胰腺病理组织学评分均显著高于A组,B组24h生存率显著低于A组。结论微量输液泵应用于逆行胰胆管注射牛黄胆酸钠大鼠重症急性胰腺炎造模具有可靠、简便、成功率高的优点,且病变均一性较好。Objective To study the advantages of mircroinfusion pump applied in retrograde biliopancreatic duct injection of sodium taurocholate in the induction of severe acute pancreatitis （SAP） model in rats. Methods 108 healthy male wistar rats were randomly di- vided into 3 groups： Group A, Group B and Group C, 36 rats in each, 18 out of 36 were taken for survival rate observation and the other 18 for other index observation at the time point of 3h, 6h and 12h; hand bolus retrograde injection of sodium taurocholate into biliopan- creatic duct through duodenal wall was made in Group A to induce SAP model while microinfusion pump was applied in Group B; rats in Group C underwent sham operation （open and shut） only; blood samples and prancreatic tissue were collected at the time points of 3h, 6h and 12h, and serum amylase level was measured and histopathologic grading of the pancreatic tissue was made; the survival rates in 24 hours were observed. Results The serum amylase levels and the histopathologic grading scores of Group A and of Group B were obviously higher than those of Group C; the serum amylase levels and the histopathologic grading scores of Group B at each time point were obviously higher than those of Group A while its survival rate in 24 hours were obviously lower than those of Group A. Conclu- sions The application of microinfusion pump in retrograde biliopancreatic duct injection of sodium tanrocholate is reliable, simple and of high rate of success in the induction of SAP model in rats and can provide better uniformity of lesions.

关 键 词：微量输液泵 逆行胰胆管注射 牛黄胆酸钠 大鼠 重症急性胰腺炎 造模 

分 类 号：R332[医药卫生—人体生理学]