何首乌高压蒸制法蒸制时间对何首乌抗衰老活性影响的研究  

The Study on Anti-Senility Experiment of Diploid Cells by Polygoni Multiflori Radix and its Processed Products

作　　者：王万根[1] 张宁华[2] 徐巧红 赵紫伟[1] 赵荣华[1] 俞捷[1] 

机构地区：[1]云南中医学院,云南昆明650500 [2]陕西中医学院,陕西咸阳712046 [3]温岭市第一人民医院,浙江温岭317500

出　　处：《云南中医学院学报》2013年第2期1-4,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(NO:81060337);2010-2011年中医药行业科研专项(NO:201107007)

摘　　要：目的通过何首乌高压蒸制和传统炮制对人胚肺二倍体细胞(HEL)抗衰老活性研究,筛选出高压蒸制法炮制何首乌的最佳炮制时间。方法在二倍体细胞的培养液中加入一定溶度的不同炮制时间和传统炮制的何首乌提取物,待细胞传至第25代以后,观察二倍体细胞的状态,检测二倍体细胞数量的变化以及细胞内超氧化物歧化酶(SOD)的含量。结果 3h制品首乌的抗细胞衰减率为38.04%,其细胞衰亡速率最为缓慢,为各供试样品中抗细胞衰减作用最为显著的炮制时间(P<0.001)。3h制品何首乌组细胞内SOD含量是(71.85±5.04)U.mL-1,显著高于对照组细胞内SOD酶的活性,提示该炮制品可显著提高细胞内SOD酶的活性(P<0.001)。结论 3 h制品何首乌可以显著延缓HEL细胞的衰老速度,提高细胞中SOD的活性,延缓二倍体细胞的老化,可确定为何首乌抗衰老作用最佳的炮制时间。Objective The anti-senility effects of Polygoni Muhiflori Radix （PMR）and its processed products including traditional processed products on human embryo lung diploid fibroblast cells were investigated in order to find the best processing time of PMR. Methods The diploid cells were counted and the SOD activity. It were tested after exposed by extracts of PMR and its processed products with different processing time including traditional processed products. Results The resistance attenuation rate of 3 h processed PMR group was 38.04% and the SOD activity in 3 h processed PMR group was （71.85±5.04）U·mL^-1. The SOD activity and the resistance attenuation rate of diploid cells in 3 h processed PMR group were significantly higher than the control group （P〈 0.001 ）. Conclusion 3 h processed PMR could delay the decline of human embryonic lung ceils while increase SOD activity in HEL cells. Thus, the most apporatiate processing time of PMR was 3 hours high-pressure steaming.

关 键 词：何首乌 炮制 人体胚肺二倍体细胞 抗衰老 

分 类 号：R283.1[医药卫生—中药学]