针刺“内关”、“心俞”穴对冠状动脉粥样硬化性心脏病模型大鼠MMP-9基因表达的影响  

Effects of Acupuncture Neiguan(PC 6)and Xinshu(BL 15)on the Expression of MMP-9 with Coronary Heart Disease Rats

作　　者：孙旭[1] 李梦[2] 沈德凯[1] 胡玲[1] 蔡荣林[1] 吴子建[1] 韦志强[2] 彭传玉[2] 

机构地区：[1]安徽中医学院针灸经络研究所,安徽合肥230038 [2]安徽中医学院,安徽合肥230038

出　　处：《云南中医学院学报》2013年第2期5-9,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(NO:81102659);安徽省高等学校自然科学研究项目(NO:KJ2011A188);针灸基础与技术安徽省重点实验室开放基金资助项目(NO:2009zjkf001A)

摘　　要：目的探讨针刺"内关"、"心俞"穴对冠心病的治疗作用及其机制,为临床防治冠心病和腧穴配伍作用提供实验依据。方法清洁级健康雄性Wistar大鼠75只,按照随机抽样的方法选择15只作为正常对照组,其余大鼠饲喂高脂饲料复制冠心病模型,随机选择15只大鼠作为针刺预处理组,在模型复制的同时予针刺"内关"、"心俞"干预治疗。其余模型复制成功的大鼠随机分为模型对照组、针刺治疗组、阳性药物对照组,每组15只。针刺治疗组选取"内关"和"心俞"进行针刺治疗,药物对照组予以阿托伐他汀混悬液灌胃,治疗两周。实验结束后,HE染色观察大鼠冠状动脉脂质沉积情况;采用荧光定量PCR法检测冠状动脉组织MMP-9 mRNA表达情况。结果模型复制成功后,模型组大鼠冠状动脉组织脂质沉积及MMP-9 mRNA表达量明显高于正常对照组(P<0.01)。而与模型组相比较,针刺预处理组、针刺治疗组和阳性药物对照组大鼠冠状动脉组织脂质沉积及MMP-9 mRNA表达显著减少(P<0.01);且针刺治疗组与阳性药物对照组比较差异均有统计学意义(P<0.05)。结论电针"内关、心俞"组穴可以有效地降低CHD模型大鼠冠状动脉内壁脂质沉积量,同时能够减少MMP-9 mRNA表达水平,金属基质蛋白酶-9可能是针刺防治冠状动脉粥样硬化性心脏病的重要目标物质之一。Objective To investigate the effects and mechanism of acupuncture ＂Neiguan＂, ＂Xinshu＂ acupoints for treating coronary atherosclerotic heart disease,and provide experimental support for the clinical treatment and the combination effect of acupoints theory. Methods 75 clean healthy male Wistar rats, 15 of them were randomly selected as the normal control group,and the remaining rats were fed with a high fat diet to duplicate the atherosclerotie coronary heart disease model,at the beginning of this, chose 15 of them as the acupuncture preconditing group randomly, after the model successfully duplicated, the rats were randomly divided into model group, acupuncture group, and the positive control group, in each group, 15 rats were counted to make an analysis. In the 2 acupuncture group, ＂Neiguan＂ and ＂Xinshu＂ were selected to acupuncture, and in the drug control group, rats were received atorvastatin suspension orally 2 weeks. At the end of the experiment,To take the aortic arch organization,fixed at 10% of formaldehyde solution, and embedded in paraffin, cut into slices in the same section, and hematoxylin-eosin（HE ）staining such slices, then to observed the pathological morphological change and to detect the expression of MMP-9 in the sample of coronary arterial tissue by PCR. Results After model duplicated successfully, there were changes in vascular inner membrane of intima rough, fiber cap formation, and foam cell deposit in the aortic tissue of rats in model control group. Compared with in model control group, such items in acupuncture predicting group,acupuncture treating group and drugs control group were all decreased significantly （P〈0.01）. Compared with in acupuncture treating group,the atovastatin could decrease expression of MMP-9 significantly （P〈0.05）. Compared with in acupuncture predicting group,the atovastatin could decrease expression of MMP-9 not significantly （P〉0.05）. Conclusion Acupuncture at ＂Neiguan＂ and ＂Xinshu＂ acupoints can effectively prevent a

关 键 词：内关 心俞 冠状动脉粥样硬化 金属基质蛋白酶-9 

分 类 号：R245.9[医药卫生—针灸推拿学]