甲状腺激素对兔面神经再生的实验研究  

Experimental study of thyroid hormone in facial neurotization of rabbit

作　　者：张恒[1] 鄢斌成 骆文龙[2] 

机构地区：[1]四川省自贡市第一人民医院耳鼻咽喉头颈外科,643000 [2]重庆医科大学附属第二医院耳鼻咽喉头颈外科,400010

出　　处：《检验医学与临床》2013年第8期933-935,共3页Laboratory Medicine and Clinic

摘　　要：目的建立面神经横断损伤、损伤局部硅胶管套接给药的动物模型,研究甲状腺激素对再生面神经的作用。方法成年健康新西兰家兔25只,设立正常对照组、手术对照组、T3治疗组三个实验组。正常对照组随机选取新西兰家兔5只,不做任何处理,直接取面神经组织检测。选用新西兰家兔20只,制作双侧面神经横断损伤、损伤局部硅胶管套接的动物模型;其中右侧为T3治疗组,硅胶管中注入甲状腺素溶液16～18μL;左侧为手术对照组,硅胶管中注入等量生理盐水。在术后4、6周两个时间点取再生面神经,电镜检测有髓轴突比率和髓鞘厚度。结果随机薄片电镜结果显示正常对照组有髓轴突比率为90.68%±1.16%,g比率(有髓轴突直径/有髓纤维直径)为0.60±0.01。术后4周,T3治疗组的有髓轴突比率为35.96%±2.64%,而手术对照组为16.87%±1.11%;手术对照组的g比率0.80%±0.02%远远大于T3治疗组0.61%±0.02%。术后6周,T3治疗组有髓轴突比率为43.90%±1.58%,g比率为0.59%±0.01%;手术对照组有髓轴突比率为23.42%±1.31%,g比率为0.74%±0.02%。电镜结果显示在术后4周和6周,T3治疗组的有髓轴突比率和髓鞘厚度均大于手术对照组,差异有统计学意义(P<0.01)。结论面神经横断损伤后,再生室中注射T3能够促进面神经有髓轴突比率和髓鞘厚度的增加。Objective To establish animal models which allows a local administration of thyroid hormones in a closed system, and investigate the effect of thyroid hormone in the regeneration of facial nerve. Methods 25 Newze land adult rabbit with the same age of 6-8 months were selected and divided into normal control group, surgery con- trol group and T3-treated group. Five rabbit in normal control group were taken facial nerve without any treatment. 20 Newzeland adult rabbit were selected randomly, and the effect of a single and local treatment with triiodothyro- nine （T3） on axonal growth across a gap between sectioned ends of facial nerve within silicone chambers was exam- ined. After nerve transection and surgical implantation, silicone chambers were filled with either a neutral PH solu- tion of triiodothyronine dissolved in NaOH or with sterile solvent as control. 4 and 6 weeks after surgery, the regen- erated facial nerve was detected by electron microscope. Results Electron microscope result demonstrated that the myelinated axons rate in normal control group was 90.68 % ± 1.16 %, g-rate （myelinated axons diameter/myelinated fibre diameter） was 0.60%±0.01%. 4 weeks after operation, the myelinated axons rate of thyroid hormone treat- ment group was 35.96%±2.64%, and that of surgery control group was 16.87%±1.11%. The g-rate in surgery control group （0.80%±0.02%） exceeded the g-rate in the T3-treated group （0.61%±0.02%）. 6 weeks after op- eration, the myelinated axons rate was 43.90% ± 1.58% and g-rate was 0.59 % ± 0.01 % in T3-treated group. In surgery control group, the myelinated axons rate was 23.42%±1.31% and g-rate was 0.74%±0.02%. In a word, the electron microscope result discovered significantly higher percentage of myelinated axons in T3-treated group compared with surgery control group, and the myelinated axons had thicker myelin sheaths. T-test showed signifi- cant difference between the two groups （P〈0.01）. Conclusion Our research revealed that a single and local admin- istra

关 键 词：甲状腺激素 面神经再生 面神经横断损伤 

分 类 号：R651.3[医药卫生—外科学]