柑橘绿霉病拮抗细菌的筛选及其抑制效果  

Screening of antagonistic bacteria against citrus green mold and its inhibitory effects

作　　者：安国栋[1] 洪鹏[1] 胡美英[1] 魏娜[1] 陈牧宇[1] 

机构地区：[1]华南农业大学农药与化学生物学教育部重点实验室,广州510642

出　　处：《华中农业大学学报》2013年第3期52-56,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31071713)

摘　　要：从根际土壤中筛选出1株对柑橘绿霉病菌Penicillium digitatum Sacc.具有较强拮抗活性的菌株HA-01。采用平板对峙法,测定了该菌株对柑橘绿霉病菌、柑橘青霉病菌Penicillium italicum、柑橘酸腐病菌Geotri-chum candidum等12种果蔬病原菌的拮抗活性。结果表明,菌株HA-01对12种供试病原菌均有不同程度的拮抗作用,表现出广谱抗菌活性。菌落形态观察、生理生化特性分析、16S rDNA序列同源性比对以及特异性PCR检测结果表明,该菌株为解淀粉芽孢杆菌Bacillus amyloliquefaciens。对马水橘的活体接种试验表明,菌株HA-01的发酵液、菌悬液和发酵滤液处理3 d后柑橘绿霉病的发病率均低于20%,病斑直径均在2.00 cm以下,而对照发病率为94.44%,病斑直径为3.60 cm。A bacterial strain HA-01, isolated from rhizosphere soil, exhibited extensive antagonistic effect on 12 fruit and vegetable pathogens. The strain was identified as Bacillus amyloliquefaciens based on morphological observation, physiological and biochemical characteristics, 16S rDNA sequence homology and specific PCR detection. The effects of different treatments of strain HA-01 against disease caused by Penicillium digitaturn Sacc. on inoculated mashui mandarin were also investigated. The re- sults showed that the incidence of citrus green mold of mashui mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HA-01 was less than 20% in all treatments while the incidence of control was 94. 44%, and the lesion diameter was all below 2. 00 cm while the lesion diameter of the control was 3.60 cm.

关 键 词：柑橘绿霉病菌 拮抗细菌 解淀粉芽孢杆菌 抑制效果 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]