硝磺草酮降解菌的分离鉴定及其降解特性  

Isolation,identification and characterization of a mesotrione-degrading bacterial strain

作　　者：韩海涛[1] 刘婕[1] 高云飞[1] 张建英[1] 钟国华[1] 

机构地区：[1]华南农业大学昆虫毒理研究室,广州510642

出　　处：《华中农业大学学报》2013年第3期62-66,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30871660)

摘　　要：采用富集培养法,从活性污泥中分离到硝磺草酮降解菌HZ-2菌株,通过形态、生理生化特征及16SrDNA序列分析,初步鉴定其为短小芽孢杆菌Bacillus pumilus。在通气、pH 7.0、温度30℃、1%接种量、摇速180r/min、50 mL LB培养基条件下共培养5 d,该菌株对200 mg/L硝磺草酮的降解率达到100%。试验结果表明,分离到的硝磺草酮降解菌HZ-2菌株能高度耐受并快速降解硝磺草酮。A bacterial strain HZ-2 was newly isolated by enrichment culture from the activated sludge in a sewage treatment plant. Based on the morphology, the physiological and biochemical charac- teristics, and 16S rDNA sequence analysis,the strain HZ-2 was identified as Bacillus pumilus HZ-2. The strain HZ-2 could not only completely degrade mesotrione 200 mg/L in 5 d, under the conditions of 1% inoculum, 30 ℃, pH 7.0 and 180 r/min, but also could tolerate and efficiently degrade mesotrione of high concentrations up to 400 mg/L. All these results indicate the strain HZ-2 is deserved to be further developed as a candidate mesotrione-degrading microbe.

关 键 词：硝磺草酮 生物降解 短小芽孢杆菌 HZ-2菌株 

分 类 号：S482.4[农业科学—农药学]