经颅和经脊髓重复磁刺激对脊髓半横断大鼠运动功能的作用  

Effects of Repetitive Transcranial Magnetic Stimulation and Magnetic Stimulation over Spinal Cord on Motor Function Recovery after Spinal Cord Hemisection in Rats

作　　者：潘钰[1] 汪璇[2] 刘萍[2] 陈赞[3] 王玉兰[4] 

机构地区：[1]北京康复中心康复医学科,北京市100144 [2]首都医科大学生理学系,北京市100069 [3]首都医科大学宣武医院神经外科,北京市100053 [4]首都医科大学宣武医院中心实验室,北京市100053

出　　处：《中国康复理论与实践》2013年第4期324-328,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市自然科学基金(No.7123216);首都医科大学基础临床合作课题(No.2007JL12)

摘　　要：目的观察经颅和经脊髓重复磁刺激对脊髓半横断大鼠运动功能的影响。方法建立T10大鼠脊髓半横断模型,脊髓半横断大鼠于术后4 d分别给予经颅和经脊髓重复磁刺激治疗,刺激强度为最大输出强度的35%,刺激频率5 Hz,每序列5 s,间歇2 min,连续10个序列,每天1次,每周5 d,连续2周。各组大鼠治疗前和治疗后进行BBB评分和水平梯子实验评价运动功能;术后38 d取患侧下肢胫前肌行降钙素基因相关肽免疫组织化学染色。结果经颅磁刺激组大鼠手术17 d后各时间点BBB评分和水平梯子实验步态正确率高于术后3 d和脊髓损伤组(P<0.05);经脊髓磁刺激组大鼠手术10 d后各时间点BBB评分和步态正确率高于术后3 d和脊髓损伤组(P<0.05),术后10 d步态正确率高于经颅磁刺激组(P<0.05)。经颅和经脊髓磁刺激组大鼠胫前肌运动终板降钙素基因相关肽表达显著高于脊髓损伤组(P<0.001)。结论早期经颅和经脊髓磁刺激可促进脊髓半横断大鼠运动功能改善,并改变远端肌肉的可塑性,经脊髓磁刺激可加速脊髓半横断大鼠运动功能恢复。Objective To observe the effect of repetitive transcranial magnetic stimulation （rTMS） and functional magnetic stimulation over spinal cord （SC-MS） on motor function recovery after spinal cord hemisection in rats. Methods T^0 spinal cord hemisection model was made. The stimulation （5 ~ 10 s bursts of 5 Hz at 35% maximal stimulator output, each burst separated by a 2 m interval） was delivered daily, 5 d per week for 2 weeks. The treatment began at 4 d after surgery for rTMS group and SC-MS group. Motor function recovery was as- sessed with Basso, Beattie ＆ Bresnahan locomotor rating scale （BBB） and the Horizontal Ladder test. The tibialis anterior was surgically re- moved at 38 d after spinal cord injury for calcitonin gene-related peptide （CGRP） iummunohistochemical staining. Results The scores of BBB and Horizontal Ladder test were significantly more at 17 d after spinal cord hemisection in rTMS group than before treatment and in spinal cord injury group （P〈0.05）. In SC-MS group, the scores of BBB and Horizontal Ladder Test were significantly more l0 d after SCI than before treatment and in SCI group （P〈0.05）. The score of Horizontal Ladder test of SC-MS group was more l0 d after SCI than that in the rTMS gourp （P〈0.05）. The expression of CGRP on motor endplates of the tibialis anterior in rTMS group and SC-MS group were more than those of SCI group （P〈0.01）. Conclusion rTMS and SC-MS in acute stage can improve the motor function recovery and muscle plastic- ity after spinal cord hemisection in rats. The magnetic stimulation can facilitate the recovery of motor function after spinal cord hemisection in rats.

关 键 词：经颅磁刺激 经脊髓磁刺激 脊髓半横断 运动功能 恢复 大鼠 

分 类 号：R651.2[医药卫生—外科学]