转化生长因子β_1/Smads通路在急性心肌梗死后心肌重塑中的作用及祛瘀生新法的干预研究  

Effect of Quyushengxin Method on Transforming Growth Factor-β_1/Smads in Ventricular Remodeling of Rats after Acute Myocardial Infarction

作　　者：张秀静[1] 赵海滨[1] 王帅[2] 郭梦[2] 许晓英[2] 马迪[2] 

机构地区：[1]北京中医药大学第三附属医院,北京市100029 [2]北京中医药大学,北京市100029

出　　处：《中国康复理论与实践》2013年第4期329-333,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京中医药大学自主选题项目资助(No.2011JYBZZJS-026)

摘　　要：目的通过研究祛瘀生新中药对转化生长因子(TGF)-β1/Smads通路的影响,探讨祛瘀生新法对急性心肌梗死后心肌重塑的效应机制。方法清洁级雄性Wistar大鼠50只随机分为正常组、假手术组、模型组、中药组、西药组,每组10只。中药组大鼠术前3 d给药,1次/d。7 d后,检测外周血骨髓间充质干细胞(BMSCs)含量,实时荧光定量RT-PCR检测心肌组织TGF-β1mRNA、Smad3 mRNA、Smad7 mRNA表达,并观察心肌组织HE染色结果及免疫组化检测心肌c-kit细胞数。结果模型组较假手术组心肌组织TGF-β1mRNA、Smad3 mRNA表达均增加(P<0.05),Smad7 mRNA表达降低(P<0.05)。中药组心肌组织TGF-β1mRNA、Smad3 mRNA表达降低,Smad7 mRNA表达增加,中药组及西药组较模型组c-kit细胞数明显增多,并能减轻心肌组织的病理损伤。中药组与西药组比较,TGF-β1mRNA、Smad3 mRNA和Smad7 mRNA表达无显著性差异(P>0.05)。结论祛瘀生新法能有效抑制急性心肌梗死后心肌重塑。Objective To explore the effect of Quyushengxin Method on transforming growth factor-13,/Smads in ventricular remodeling of rats after acute myocardial infarction. Methods 50 male Wistar rats were randomly divided into control group, sham operation group, myocardial infarction group, Chinese medicine group and western medicine group with 10 rats in each group. Chinese medicine group was administered 3 d before the operation once a day. The rats were sacrificed 7 d later. The peripheral blood bone marrow mesenchymal stem cells （BMSCs） was detected and the mRNA level of TGF-B1 Smad3 and Smad7 were observed with RT-PCR. Myocardial c-kit cell number and HE staining results were also observed. Results The expression of TGF-B1 mRNA and Smad3 mRNA were higher and the expression of Smad7 mRNA was lower in the model group than in the sham operation group （P〈0.05）. The expression of TGF-B1mRNA and Smad3 mRNA were lower and the expression of Smad7 mRNA was higher in the Chinese medicine group than in the model group （P〈0.05）. And the c-kit cell number was more in both Chinese medicine group and western medicine group than in the model group. And there was no sig- nificant difference between Chinese medicine group and western medicine group （P〉0.05）. Conclusion Quyushengxin Method can inhibit ventricular remodeling after myocardial infarction in rats.

关 键 词：祛瘀生新法 急性心肌梗死 心肌重塑 转化生长因子-β1/Smads 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]