转化生长因子β1在强直性脊柱炎患者黄韧带和椎旁肌中的表达  

Expression of transforming growth factor-β1 in ligamentum flavum and paraspinal muscle in patients with ankylosing spondylitis

作　　者：许红飞[1] 初同伟[1] 张超[1] 周传奇[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《中国免疫学杂志》2013年第4期344-346,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金(81071523);重庆市重点项目(CSTC2011BA5009)资助

摘　　要：目的:探讨转化生长因子β1(Transforming growth factorβ1,TGF-β1)在强直性脊柱炎(Ankylosing spondylitis,AS)黄韧带、椎旁肌中的表达,探讨其在组织纤维化中的意义。方法:通过免疫组化检测TGF-β1在黄韧带及椎旁肌中的表达,用图像分析系统比较它们的表达差异。通过HE、Massoon、VG染色,观察并统计它们的纤维化改变及程度。结果:TGF-β1在实验组中阳性表达,黄韧带及椎旁肌纤维化改变程度重;对照组无明显TGF-β1表达,黄韧带及椎旁肌未发现纤维化改变。两组TGF-β1表达的光密度、黄韧带弹性纤维/胶原纤维、椎旁肌肌纤维/胶原纤维差异有统计学意义(P<0.01)。结论:AS患者黄韧带、椎旁肌高表达TGF-β1,促进组织的纤维化过程。Objective ：To examine the expression and effect of transforming growth factor-β1 in ligamentum flavum and paraspi- nal muscle in patients with ankylosing spondylitis （AS）. Methods：Expression of TGF-β1 in ligaments was tested by immunohisto- chemistry, in order to discriminate the changes of fibrosis with HE, Masson, VG stain. Results ：TGF-β1 highly expressed in ligamen- tum flavum and paraspinal muscle in AS group, and no expression was detected in control group. The level of fibrosis in AS group was obvious, but the control group showed no TGF-β1 expression and had no sign of fibrosis. Statistical difference existed in the integrated optical density of TGF-β1, ration of elastic/collagen, ration of muscle/collagen （P 〈 0.01 ）. Conclusion： TGF-β1 was highly expressed in ligamentum flavum and paraspinal muscle in ankylosing spondylitis, which may promote the change of tissue fibrosis.

关 键 词：强直性脊柱炎 转化生长因子Β1 黄韧带 椎旁肌 

分 类 号：R593.23[医药卫生—内科学] R392.31[医药卫生—临床医学]