SSeCKS调节激活的星形胶质细胞炎症因子TNF-α和NO的释放  

SSeCKS regulated the relese of inflammatory factor TNF-α and NO in activated astrocytes

作　　者：李晓红[1] 戚磊[2] 郭益冰[1] 陆晶晶[1] 朱慧[1] 吴信华[1] 鞠少卿[3] 

机构地区：[1]南通大学附属医院外科综合实验室,南通226001 [2]南通大学计算机科学与技术学院,南通226001 [3]南通大学附属医院医学检验中心,南通226001

出　　处：《中国免疫学杂志》2013年第4期350-353,共4页Chinese Journal of Immunology

基　　金：国家自然科学青年基金(81200918);南通大学校级自然科学基金(11Z012)的资助

摘　　要：目的:星形胶质细胞是CNS中主要的胶质细胞类型,维持着CNS内环境的稳态。慢性激活的星形胶质细胞被认为参与了包括MS在内的神经免疫学疾病。SSeCKS在EAE病程中表达显著上调,并且主要表达于神经元和胶质细胞中,参与EAE炎症的调节。本实验探讨SSeCKS对星形胶质细胞炎症因子释放的影响,为MS和EAE的治疗寻找新的靶点。方法:本实验通过培养原代星形胶质细胞,脂质体介导SSeCKS基因沉默,并采用ELISA检测星形胶质细胞中肿瘤坏死因子-α以及一氧化氮的分泌情况。结果:原代培养的星形胶质细胞纯度达到了95%以上,SSeCKS干扰后星形胶质细胞炎症因子分泌显著降低。结论:SSeCKS参与星形胶质细胞炎症因子的分泌,可能为MS和EAE治疗提供新的思路。Objective：Astrocytes is the majoy glial cell type in the CNS, which maintain the environment of CNS. Chronic activation of astrocytes is thought to be involved in neurological autoimmune diseases, such as MS. In preview report, SSeCKS expression was significantly up-regulated in EAE, and was expressed in neurons and glial cells. In order to find new targets for the treatment of MS and EAE, the relationship between release of inflammatory cytokines in astrocytes and the exprssion of SSeCKS was studied. Methods ： Primary cultures of astrocyte and oligodendrocyte were isolated from postnatal day 0-1 Lewis rats. SSeCKS-siRNA and nonspecific-siRNA plasmids were transfected into astrocytes. Cytokines secreted from astrocyte were detected by sandwich enzyme-linked immunosorbent assay （ELISA） in order to examine the role of SSeCKS in cytokines secreted of astrocyte, induced by LPS or LPS/IFN-γ. Resuits： TNF-α and NO were induced by LPS or LPS/IFN-α, in primary astrocytes in vitro. SSeCKS was involved in TNF-α and NO secretion in primary astrocytes. Conclusion： Transient transfection of SSeCKS-siRNA suppressed LPS or LPS/IFN-γ,-stimulated TNF-α and NO secretion in astrocytes. Understanding the mechanism of SSeCKS in the pathogenesis of EAE may contribute to the development of new therapeutic strategies against EAE and MS.

关 键 词：星形胶质细胞 SSECKS 肿瘤坏死因子 一氧化氮 

分 类 号：R392.9[医药卫生—免疫学]