CXCR1/2受体拮抗剂-G31P对小鼠腹水型肝癌淋巴转移潜能的影响  

Effect of CXCR1/2 antagonist on lymphatic metastasis of ascites hepatocarcinoma in mouse

作　　者：彭静[1] 王冰[2] 魏晶[2] 王世仪[2] 屈虹[2] 李芳[2] 

机构地区：[1]新疆克拉玛依市中心医院检验科,克拉玛依834000 [2]大连医科大学基础医学院免疫教研室,大连116027

出　　处：《中国免疫学杂志》2013年第4期386-391,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(No.30772023)

摘　　要：目的:建立小鼠腹水型肝癌淋巴道高转移细胞系HCA-P/L6足趾转移动物模型,观察CXCR1/2受体拮抗剂-G31P对该模型转移潜能的影响。方法:用小鼠腹水型肝癌淋巴道高转移细胞系HCA-P/L6制造足趾转移动物模型。同时背部皮下注射CXCR1/2受体拮抗剂G31P或生理盐水作为实验组和对照组。肉眼观察转移模型中小鼠足垫原位移植瘤出现的时间和瘤体的状态、淋巴结肿大情况,记录小鼠健康状况。第21天时,解剖小鼠,取各部位淋巴结并测量其最长径,并取足垫皮下原位移植瘤及可疑转移脏器,制作病理切片。切取部分淋巴结RT-PCR检测淋巴结中CXCR2、KC、MIP2 mRNA的表达水平。结果:两组小鼠体重和原位移植瘤体积即足趾体积比较具有显著性差异(P<0.05)。对照组小鼠双侧腹股沟淋巴结、腋窝淋巴结、主动脉旁淋巴结直径均大于实验组(P<0.05),且转移率有显著差异,病理检测显示对照组小鼠各部位淋巴结瘤细胞呈片状分布,异性型明显,并可见多核的巨瘤细胞。实验组小鼠淋巴结中只有一小部分可见个别散在瘤细胞。RT-PCR显示对照组小鼠足垫原位移植瘤MIP-2 mRNA表达明显增高。结论:CXCR1/2受体拮抗剂-G31P可以有效抑制小鼠腹水型肝癌淋巴转移。Objective：To establish animal models with murine hepatocarcinoma cell line with high lymphatic metastatic potential HCA-P/L6 and observe CXCR1/2 antagonist-G31P metastatic potential of the model. Methods： The routine hepatocarcinoma cell line with high metastatic lymphatic potential （HCA-P/L6） was subcutaneously inoculated into the medioventral line of mice 615 and the second passage ascites hepatoearcinoma cells were obtained, taken ascites cell count, diluted with normal saline to the concentration of 4 × 10^7ml 1. Mice were inoculated with the second passage HCA-P/L6 cells in posterior left foot, while subcutaneous injection of G31P or saline as the treated group and control group. Naked eye observed the model in situ mouse the time of footpad tumor appearance and the status of tumor, lymph node enlargement, recorded the health status of mice. On 21st day, dissected the mouse, took lymph node of different parts and measured the longest diameter, and got the in situ transplanted subcutaneous tumor and suspected organs, produced slides for pathology. Excised some lymph nodes for RT-PCR to detect the expression of CXCR2, KC, MIP2 mRNA. Re- sults ：The body weight and the volume of in situ transplanted tumor of the groups are different, with significant difference （ P 〈 0.05 ）. Spresding rate also has a significant difference. The pathological examination of lymph node of control group showed that heteromorphism is obvious, and muhinucleated tumor cells have been widely seen. And only a small part of the visible individually scattered tumor cells in the treated group mice. RT-PCR showed the expression of MIP-2 mRNA of foot pad in situ tumor in the control group mice was significantly increased. Conclusion：CXCR1/2 antagonist-G31P can inhibit the murine hepatoearcinoma lymphatic metastasis.

关 键 词：腹水型肝癌 CXCR1 2 转移 

分 类 号：R392.9[医药卫生—免疫学]