SPA预处理对小鼠金葡菌致死性攻击的保护作用  

Pretreatment with SPA protects mice from lethal challenged with live Staphylococcus aureus

作　　者：侯晓睿[1] 陈益国[2] 朱平[1] 刘北一[1] 富宁[1] 

机构地区：[1]南方医科大学基础医学院免疫教研室,广州510515 [2]江西省人民医院检验科.南昌330006

出　　处：《中国免疫学杂志》2013年第4期403-406,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(项目号31270980);广东省自然科学基金(S2012010009562)资助

摘　　要：目的:探讨金葡菌A蛋白(SPA)预处理小鼠对金葡菌致死性攻击的保护作用。方法:于0、24小时对BALB/c小鼠行腹腔注射SPA 20μg/只/次,对照组以生理盐水替代SPA。第二次处理24小时后,腹腔注射致死量(3×1010CFU/kg)金葡菌(ATCC25923),观察小鼠生存率;非致死量金葡菌(2×108CFU/只)腹腔感染6小时后,ELISA、Q-PCR检测小鼠血清及脾脏中TNF-α、IL-6、IFN-γ含量及表达量。结果:与对照组小鼠2天内全部死亡比较,致死量细菌攻击后,SPA预处理组小鼠2周生存率为30%,显著高于对照组(P<0.05);非致死量细菌感染后,预处理组小鼠血清及脾脏中TNF-α、IL-6、IFN-γ含量及表达量显著低于对照组(P<0.05),IL-10表达量降低。结论:SPA预处理增强BALB/c小鼠抵御S.aureus致死攻击。Objective： To study the protective effect of SPA pretreatment on mice challenged with lethal-dose Staphylococcus aureus. Methods ： BALB/c mice were given intraperitoneal injections （ i. p） of 20 μg SPA or normal saline at 0 h and 24 h on consecutive 2 days. All of mice were then challenged by an i. p injection of live Sthaphylococcus aureus （ATCC25923） with lethal-dose （3×10^10CFU/kg） at 1 day after the second pretreatment. The mice mortality was observed, and the serum level of IFN-γ, TNF-α and IL- 6 in mice infected with non-lethal live Staphylococcus aureus （2×10^8 CFU for each, i. p） were detected by ELISA. Meanwhile, the gene relative expression of these cytokines in splenocyte was detected by Q-PCR. Results： Compared with normal saline treated, the pretreated with SPA prolonged significantly the survival of mice challenged with lethal live S. aureus （ P 〈 0.05 ）. The level and gene relative expression of TNF-α, IL-6, IFN-γ and IL-10 of serum and splenocyte in mice that were administrated with SPA were reduced （ P 〈 0. 05 ）. Conclusion ： Pretreatment with SPA protects the mice from challenge with lethal and non-lethal live S. aureues.

关 键 词：金葡菌 金葡菌A蛋白 感染 细胞因子 

分 类 号：R392[医药卫生—免疫学]