α粒子诱发人支气管上皮细胞转化不同时期miR-200家族表达的研究  

Study on MiR-200 Family Expression in the Stages of Transformation of Human Bronchial Epithelial Cells Induced by α-Particles

作　　者：高刚[1] 杨英杰[1] 刘建香[1] 田梅[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中国辐射卫生》2013年第2期135-137,共3页Chinese Journal of Radiological Health

基　　金：卫生行业科研专项(201002009)

摘　　要：目的研究α粒子诱发人支气管上皮细胞(BEP2D)转化不同时期miR-200家族表达变化情况。方法用实时荧光定量PCR检测miR-200家族和ZEB家族及上皮间质转变相关标记分子在α粒子诱发BEP2D转化细胞中的表达变化情况。结果与BEP2D和R15H20细胞相比,RHT35细胞中miR-200家族的表达显著下调(P<0.01);与BEP2D和R15H20细胞相比,RHT35细胞中ZEB1和ZEB2的表达显著上调,P<0.01;与BEP2D和R15H20细胞相比,RHT35细胞中上皮-钙连接素mRNA的表达显著下调,神经-钙连接素mRNA的表达显著上调,P<0.01。结论α粒子诱发人支气管上皮细胞转化不同时期miR-200家族表达发生显著改变。Objective To investigate miR -200 family expression in the stages of transformation of human bronchial epithelial cells induced by α - particles. Methods Real Time PCR was used to detect miR - 200 family and ZEB family and epithelial mesenchymal transition marker expression in transformed cells induced by α - particles. Results The downregulation of mature miR-200a, miR -200b, miR -200c and miR - 141 expression in RHT35 cells was detected by Real Time PCR compared with R15H20 and BEP2D cells. The mRNA level of ZEB1 and ZEB2 in RI-IT35 cells upregulate compared with R15H20 and BEP2D cells, P 〈0.01. The mRNA level of E -cadherin in RHT35 cells downregulate and N -cadherin upregulate compared with R15H20 and BEP2D cells, P 〈0.01. Conclusion MiR -200 family are differentially expressed in the stages of transformation of human bronchial epithelial cells induced by α particles.

关 键 词：Α粒子 人支气管上皮细胞 miR-200家族 

分 类 号：Q345.1[生物学—遗传学]