金纳米粒子分光光度法测定尿中8-羟基脱氧鸟苷  

Determination of urinary 8-hydroxy-2'-deoxyguanosine using gold nanoparticles by spectrophotometry

作　　者：王嘉成[1] 王永生[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2013年第4期745-747,共3页Applied Chemical Industry

基　　金：湖南省科技厅科技计划资助项目(2010SK3039)

摘　　要：在pH为3.5的BR缓冲溶液中,8-羟基脱氧鸟苷(8-OHdG)与金纳米粒子作用,导致溶液吸光度比值改变,且在0.498×10-6～12.7×10-6mol/L范围内,吸光度值改变与8-OHdG的浓度呈良好的线性关系,其回归方程为:ΔA630/A520=0.081 2c-0.007 13,相关系数r=0.998 4,检出限为0.150×10-6 mol/L,相对标准偏差(RSD)在1.48%～3.57%。用于尿中8-OHdG的测定,回收率在96.1%～98.0%。该方法简单、快速、成本低。In the Britton-Robinson buffer solution of pH 3.5,8-hydroxy-2 ＇ -deoxyguanosine （8-OHdG） combined with gold nanoparticles, resulting in absorbance change of the solution. A linear correlation existed between the absorbance change and the concentration of 8-OHdG in the range of 0. 498 × 10-6 - 12.7 ×10 -6 mol/L. The equation of linear regression is △A630/A520 = 0.081 2c -0.007 13 with a correlation coefficient （r） of 0. 998 4. The detection limit （LOD） is 0. 150 × 10-6 mol/L. The relative standard deviations were 1.48% - 3.57%, and the recovery was 96.1% - 98.0%. The proposed method was simple, rapid and cheap.

关 键 词：8-羟基脱氧鸟苷 金纳米粒子 分光光度法 

分 类 号：TQ203.9[化学工程—有机化工] O657.32[理学—分析化学]