宁夏致泻性大肠杆菌的流行特征及耐药现状研究  

The epidemiological characteristics and current status of the drug resistance of diarrheagenic escherichia coli in Ningxia

作　　者：郭邦成[1] 闫立群[1] 郝琼[1] 刘翔[1] 谢明英[1] 沈梅[1] 魏琼[1] 景怀琦[2] 王鑫[2] 刘萍[3] 李玉忠 李广琴 吴惠琴[5] 

机构地区：[1]宁夏自治区疾病预防控制中心,宁夏银川750004 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]宁夏中卫市疾病预防控制中心,宁夏中卫751700 [4]宁夏青铜峡市疾病预防控制中心,宁夏青铜峡751600 [5]宁夏平罗县疾病预防控制中心,宁夏平罗753400

出　　处：《宁夏医学杂志》2013年第4期307-309,共3页Ningxia Medical Journal

基　　金：宁夏自治区卫生厅重点科研计划课题(2011058);国家"十一五"传染病科技重大专项实验室监测技术平台项目(2009ZX10004-203)

摘　　要：目的探讨宁夏地区致泻性大肠杆菌(Diarrheagenic E,Coli scherichia,DEC)菌群分布、药物敏感性情况,为腹泻病防控提供依据。方法 2010—2011年共收集宁夏医科大学总医院、银川市第二人民医院、中卫市医院、青铜峡市医院门诊及住院腹泻患者新鲜大便1 520份,接种于买康凯(MaC)培养基,挑选麦康凯琼脂平板上玫瑰红色光滑型菌落,转种TSA平板,37℃培养24 h,采用聚合酶链反应(PCR)进一步做菌株鉴定及分型;细菌药敏采用Kirby-Bauer法。结果 1 520例腹泻患者共检出98株DEC,检出率为6.45%,其中主要为PEC检出53株,占分离大肠菌株的54.08%。药敏试验结果表明,DEC对常用抗生素均产生较高的耐药性,对氨苄西林、阿莫西林和复方磺胺甲恶唑的耐药性最为严重,达到100%,庆大霉素、氯霉素和头孢噻肟的敏感性较高。结论宁夏地区致腹泻病原主要为DEC,且对常用抗生素具有较高的耐药性,应引起高度重视。Objective To discuss the distribution and drug sensitivity of diarrheagenic E coliflora in Ningxia so as to provide the ba-sis for diarrhea prevention and control. Methods The fresh stool of 1 520 diarrhea cases was obtained from General Hospital of Ningxia Medi-cal University, the Second People＇s Hospital of Yinchuan, Zhongwei People＇s Hospital and Qingtongxia People＇s Hospital, and then inoculated the stool on the Mac medium and sorted out the rose-bengal smooth flora on the Mac agar plate, adapted on TSA plate, raised in 37℃ for 24h. Strain identification and classification were made by using PCR. Antimicrobial susceptibility used the Kirby-Bauer method. Results 98 diarrheagenic E were found in 1520 patients. Coli with a positive rate was 6. 45%, EPEC was higher than other coli. gesults showed that drugs with higher resistance rate were commonly used antibiotics. The identification of strains showed that it was pathogenic E coli type. The drug resistance rates of AMP and AML OXA were more than 99%, and there was higher sensitivity to GM, C and CTX. Conclusion The EPEC of diarrheagenic coli is popular in Ningxia and there is a high resistance to commonly use antibiotics.

关 键 词：致泻性大肠杆菌 聚合酶链反应 药敏分析 

分 类 号：R378.2[医药卫生—病原生物学]