小鼠合体滋养细胞微绒毛膜制备方法的研究  

The study on a method for preparation of syncytiotrophoblast microvillous membrane of mice

作　　者：李怡琳[1] 韩健[1] 刘晓洁[1] 韩磊[1] 俞丽丽[1] 李红梅[1] 周丽娟[1] 张欣[1] 郑英如[1] 郭建新[1] 李力[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所妇产科中心,重庆400042

出　　处：《重庆医学》2013年第12期1330-1331,1335,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81270008;81170576;30901612)

摘　　要：目的建立一种制备小鼠合体滋养细胞微绒毛膜(STBM)的方法。方法收集足月孕小鼠的胎盘,利用机械分离法剪碎胎盘进行差速离心,分离小鼠STBM。用Lowry法测定制备的小鼠STBM蛋白水平,用组织多肽抗原作为小鼠STBM的标记物,采用酶联免疫吸附测定(ELISA)检测STBM的水平,利用电镜观察并分析小鼠STBM的形态结构。结果实验获得的小鼠STBM制剂的蛋白水平为(0.69±0.12)mg/mL,TPA水平为(61.49±9.78)ng/mL。电镜下小鼠STBM的直径为(228.52±90.06)nm的囊泡状结构。结论制备的小鼠STBM与人STBM高度相似,可为子痫前期致病机制的探索提供在体研究的实验基础。Objective To construct a method to prepare syncytiotrophoblast microvillous membrane（STBM） of mice. Methods STBM were prepared from placentas which were collected sterilely,immediately from full term mice. After breaking up the placenta mechanically,STBMs were separated by differential centrifugation. Lowry＇s method was used to detect total protein concentration of STBM. ELISA was applied to detect STBM. Scanning electron microscope was used to observe the morphological characteristics of STBM. Results Total protein level of the STBM prepared from mice in this study was （0.69±0.12）mg/mL. TPA level of ST-BM preparation was （61.49±9. 78）ng/mL. Under the scanning electron microscope, the diameter of STBM micro-particles was （228.52±90.06）nm. Conclusion The protein marker and morphological characteristics of STBM preparation of mice from our study were similar to the STBM preparation of human.

关 键 词：合体滋养细胞微绒毛膜 胎盘 C57BL 6小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]