自身免疫性疾病患者外周血CD8^+调节性T细胞的表达及临床意义  

Expression and clinical significance of CD8^+ regulatory T cells in peripheral blood of patients with autoimmune diseases

作　　者：陈莉[1] 胡宗海[1] 彭燕[1] 栗群英[1] 李继红[1] 冯苏娟[2] 

机构地区：[1]中国人民解放军成都军区总医院检验科,成都610083 [2]重庆日报报业集团医疗保健中心,重庆400012

出　　处：《重庆医学》2013年第12期1360-1361,1364,共3页Chongqing medicine

摘　　要：目的研究自身免疫性疾病(AID)患者外周血CD8+调节性T细胞的表达及其临床意义。方法对53例系统性红斑狼疮(SLE)患者和55例类风湿性关节炎(RA)患者及38例健康者(对照组)外周血以双抗体标记流式细胞术测定CD8+CD28-和CD8+CD28+T细胞亚群比例及CD8+CD28-/CD8+CD28+细胞间比值,分析检测数据与疾病活动变化的关系。结果 SLE患者稳定期CD8+CD28-T细胞亚群比例较活动期及对照组显著升高,差异有统计学意义(P<0.05);活动期CD8+CD28+T细胞亚群比例较稳定期和对照组显著降低,差异有统计学意义(P<0.05),CD8+CD28-/CD8+CD28+细胞间比值较稳定期和对照组显著升高,差异有统计学意义(P<0.05)。SLE活动性指数评分(SLEDAI)与CD8+CD28+T细胞亚群比例负相关(P=0.001,r=-0.56),与CD8+CD28-/CD8+CD28+比值正相关(P=0.002,r=0.51)。RA患者稳定期CD8+CD28-T细胞较活动期和对照组增高,差异有统计学意义(P<0.05),CD8+CD28+T细胞及CD8+CD28-/CD8+CD28+比值RA活动期和稳定期患者与对照组比较,差异无统计学意义(P>0.05)。结论 AID患者存在CD8+T细胞亚群比例的改变,反映机体细胞免疫功能紊乱。Objective To explore the expression and clinical significance of CD8^＋ regulatory T cells in peripheral blood of pa-tients with autoimmune diseases（AID）. Methods Peripheral blood of 53 patients with systemic lupus erythematosus（SLE） and 55 patients with rheumatoid arthritis（RA） and 38 healthy control were measured by flow cytometry. The proportion and ratio of CD8^＋ CD28^- and CD8^＋ CD28^＋ T lymphocyte subpopulations were detected. The relativity between detection data and disease activity in dex was analyzed. Results The proportion of CD8^＋ CD28^- T lymphocyte subpopulation of SLE patients in stable stage compared to the active stage and the control group was increased significantly（P〈0.05）. The proportion of CD8^＋ CD28^＋ T lymphocyte subpop-ulation in active stage was lower than the stable stage and the control group（P〈0.05）. The ratio of CD8^＋ CD28^- /CD8^＋ CD28^＋ T lymphocyte subpopulations of patients in active stage was higher than patients in stable stage and the control group （P〈0. 05）. SLEDAI had negative relationship to the proportion of CD8^＋ CD28^＋ T lymphocyte subpopulation（P=0. 001, r=-0.56） and posi-tive relationship to the ratio of CD8^＋ CD28^-/CD8^＋ CD28^＋ （P = 0. 002, r=0. 51）. The proportion of CD8^＋ CD28^- T lymphocyte subpopulation of patients with RA in stable stage was higher than the proportion of patients in active stage and the control group （P〈0.05）. The proportion of CD8^＋ CD28^＋ T lymphocyte subpopulation and the ratio of CD8^＋ CD28^- /CD8^＋ CD28^- T lymphocyte subpopulations of patients with RA in active and stable periods compared with the control group had no statistical significance（P〉 0.05）. Conclusion The change of CD8^＋ T lymphocyte subpopulation of patients with AID reflects the cellular immunity function disorders of the body.

关 键 词：自身免疫性疾病 T淋巴细胞 调节性 CD8+ 流式细胞术 

分 类 号：R446.6[医药卫生—诊断学]