缝隙连接对正常血压和自发性高血压大鼠肠系膜动脉收缩反应的影响  

The effects of gap junction on the mesenteric artery contraction activities in normotensive rats and spontaneously hypertensive rats

作　　者：蒋学伟[1] 司军强[1] 李丽[1] 赵磊[1] 魏丽丽[1] 陈新燕[1] 马克涛[1] 

机构地区：[1]石河子大学医学院生理学教研室,新疆石河子832002

出　　处：《重庆医学》2013年第12期1365-1367,共3页Chongqing medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB526600);国家自然科学基金(31260247);国家人力资源与社会保障部留学回国人员科技活动项目(2011LX003);兵团博士基金项目(2010JC16);兵团医药专项(2012BA021;2010GG33);杰出青年科技人才培养计划(2012ZRKXJQ01)

摘　　要：目的本研究通过给予KCl和PE,观察正常血压Wistar大鼠和自发性高血压大鼠(SHR)肠系膜动脉(MA)收缩反应的异同,并探讨细胞间缝隙连接在血管收缩反应中的作用。方法应用压力肌动图技术,在急性分离的Wistar大鼠和SHR的MA 2级动脉分支上,观察内皮非依赖血管收缩剂KCl和PE对血管收缩的影响以及应用缝隙连接阻断剂2-APB后对血管收缩反应的影响。结果 (1)KCl和PE可以引起浓度依赖的收缩反应,且在SHR MA上KCl和PE引起的收缩幅度高于Wistar大鼠,差异有统计学意义(P<0.05)。(2)预灌流缝隙连接阻断剂2-APB(100μmol/L)后KCl和PE引起的血管收缩均显著降低(P<0.05),其中SHR降低幅度更加显著(P<0.01)。结论内皮非依赖收缩剂KCl和PE引起的SHR MA收缩幅度增强可能是通过增强血管平滑肌细胞间缝隙连接通讯增强介导。Objective To compare the contraction activities of mesenterlc artery（MA） induced by KCI and VPL between normo tensive Wistar rats and spontaneously hypertensive rats（SHR）, and to investigate the effects of gap junction traction activities. Methods Pressure myograph was used to observe the influences of KC1 and PE（endotheli on the vascular con-al independent vaso-constrictor） on the vascular contraction activities,and the differences after applying 2-APB（gap junction blockers） in acute separa-ted MA of Wistar rats and SHR. Results （1） KC1 and PE induced concentration-dependently contraction reactions, and the con-traction amplitudes induced by KC1 and PE was higher in SHR than Wistar rats respectively, there was significant difference（P〈 0.05）. （2） After application 2-APB（100μmol/L） ,the contraction amplitudes of mesenteric artery induced by KCI and PE was obvi-ously suppressed respectively（P〈0.05） ,and more significantly in SHR（P〈0.01）. Conclusion the contraction reactions of mesen-teric artery in SHR are higher than in Wistar rats through increasing the gap junction communication among smooth muscle cells of MA in SHR.

关 键 词：大鼠 近交SHR 肠系膜动脉 2-APB 缝隙连接 收缩剂 

分 类 号：R544.1[医药卫生—心血管疾病]