正交试验优选拆分5种碱性药物的毛细管电泳法  

Enantioseparation of five basic drugs by orthogonal experimental design method

作　　者：郭雯雯[1] 付小帅[1] 崔艳[1] 姜珍[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2013年第4期281-286,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273476);辽宁省教育厅科学研究一般项目(L2012353)

摘　　要：目的以羧甲基-β-环糊精(carboxymethyl-β-cyclodextrin,CM-β-CD)为手性选择剂,用毛细管电泳法(capillary electrophoresis,CE)对5种碱性药物苯磺酸氨氯地平(amlodipine besilate)、二氧丙嗪(dioxopromethazine)、硫酸特布他林(terbutaline sulfate)、氢溴酸后马托品(homatropinehydrobromide)和盐酸美普他酚(meptazinol hydrochloride)进行对映体分离研究。方法采用正交试验设计的方法考察3个水平数背景电解质溶液的pH值、CM-β-CD浓度、缓冲盐浓度对对映体分离的影响,优化了分离条件。结果在最优分离条件下,苯磺酸氨氯地平、二氧丙嗪、硫酸特布他林、氢溴酸后马托品、盐酸美普他酚对映体达到良好分离,分离度分别为12.6、4.4、6.0、5.7、3.5。结论 CM-β-CD对5种碱性药物均具有较好的对映体选择性。Objective To establish a capillary electrophoresis （CE） method for separating amlodipine besil- ate, dioxopromethazine, terbutaline sulfate, homatropine hydrobromide and meptazinol hydrochloride. Meth- ods Carboxymethyl-β-cyclodextrin （CM-β-CD） was used as a chiral selector. The basic chemical parameters of enantioseparation, such as chiral selector concentration, buffer concentration and buffer pH, were opti- mized using an orthogonal experimental design. Results Under the optimized conditions, amlodipine besilate, dioxopromethazine,terbutaline sulfate, homatropine hydrobromide and meptazinol hydrochloride were sepa- rated with the maximum resolution factors of 12. 6,4. 4,6.0,5.7 and 3.5 ,respectively. The migration order of （S）-（-）-and （R）-（＋）-amlodipine besilate was determined by spiking the sample with non-racemic ana- lyte. Conclusions CM-β-CD has a good enantioselectivity on the five kinds of drugs; orthogonal experimen- tal design is a convenient , effective method for chiral separation.

关 键 词：羧甲基-β-环糊精 毛细管电泳法 正交试验设计 对映体分离 

分 类 号：R917[医药卫生—药物分析学]