TaqMan实时PCR与SYBR Green Ⅰ实时PCR检测HLA-B*27的比较性分析  

Comparative evaluation of TaqMan real-time PCR assay and SYBR Green Ⅰ real-time PCR assay for detecting HLA-B*27

作　　者：马红玲[1,2] 王雪萍[1] 陈凤花[1] 马荣红[1] 温晓波[1] 袁琳[1] 潘世秀[1] 胡丽华[1] 李一荣[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院检验科,湖北武汉430022 [2]华中科技大学同济医学院检验系,湖北武汉430022

出　　处：《国际检验医学杂志》2013年第7期771-772,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨TaqMan实时PCR与SYBR Green Ⅰ实时PCR检出中国汉族人群基因组标本中HLA-B*27等位基因能力的差异。方法首先采用SYBR Green Ⅰ实时PCR对243例人基因组标本中的HLA-B*27等位基因进行检测,随后采用TaqMan实时PCR对HLA-B*27进行再次检测。结果 SYBR Green Ⅰ实时PCR发现243例人基因组标本中,132例HLA-B*27阳性,111例HLA-B*27阴性,而TaqMan实时PCR检测结果显示131例HLA-B*27阳性,112例HLA-B*27阴性,两者比较差异无统计学意义(P>0.05)。结论与SYBR Green Ⅰ实时PCR一样,TaqMan实时PCR也是一种快速有效的检测中国汉族人群HLA-B*27等位基因的方法。Objective To compare the capacity of TaqMan real-time PCR assay with SYBR Green I real-time PCR assay for detecting HLA-B＊ 27 allelic gene in genomic samples from Chinese Han population. Methods HLA-B ＊ 27 allelic gene of 243 hu- man genomic samples was detected by SYBR Green I real-time PCR assay following by TaqMan real-time PCR assay. Results SYBR Green I real-time PCR assay found that 132 of 243 human genomic samples were HLA-B ＊ 27 positive and the other 111 were HLA-B＊ 27 negative. TaqMan real-time PCR assay showed that 131 of 243 human genomic samples were HLA-B＊ 27 posi- tive and the other 112 were H LA-B ~ 27 negative, there was no statistically significant difference between two methods（P〉0.05）. Conclusion TaqMan real-time PCR assay was also a rapid and efficient method for detecting HLA-B ＊ 27 allelic gene in genomie samples from Chinese Han population as well as SYBR Green I real-time PCR assay.

关 键 词：聚合酶链反应 基因 HLA抗原 

分 类 号：R440[医药卫生—诊断学]