两种因子对水稻陈年种子萌发的影响  

Effect of Two Factors on the Germination of Aged Rice Seed

作　　者：杨俊年[1] 胡廷章[1] 秦刚[1] 钟彦[1] 

机构地区：[1]重庆三峡学院生命科学与工程学院,重庆万州404000

出　　处：《吉林农业科学》2013年第2期21-26,共6页Journal of　Jilin Agricultural Sciences

基　　金：重庆市教委科技项目(KJ121106);重庆市科委自然科学基金计划资助项目(cstc2012jjA80009)

摘　　要：利用不同紫外线照射时间和不同浓度秋水仙素浸种的方法对水稻陈年种子进行处理后,通过测定其萌发率和淀粉酶活性来分析两种因子的影响。结果显示:1、紫外线照射2 h以上,可极显著降低种子萌发率,但是0.2%和0.3%浓度秋水仙素则能显著提高萌发率。两种因子不同组合处理均能极显著地促进萌发率,其中3个处理组的萌发率都高达92.22%。有秋水仙素处理并且紫外线照射2 h以上种子的萌发率并不下降。2、不同时间紫外照射都能使萌发中淀粉酶活性极显著地提高。0.2%和0.4%的秋水仙素处理使酶活性显著地提高,0.3%和0.5%浓度处理的效果极显著地高于对照组。两因子分别处理时,秋水仙素处理的效果没有紫外线好。两种因子不同组合的处理中有10个组的酶活性显著提高。大多数处理组酶活性呈现出两个高峰值,分别出现在第二天和第五天,所有的处理组均在第一天或第二天达到酶活性的最大值。Aged rice seeds were treated with different time of ultraviolet irradiation and soaked with dif ferent concentration of colchicine. The effect of two kinds of factors was analyzed by measuring seed germi nation rate and amylase activity. Results showed that more than 2 hours ultraviolet irradiation significantly reduced the seed germination rate, but 0.2% and 0.3% concentration colchicine significantly improved the germination rate. Two kinds of different combination treatments significantly promoted the germination rate. In three of the treatment combination, seed germination rate was as high as 92.22%. When seed were treated with colchicine and 2 hours of ultraviolet irradiation at the same time, germination rate did not decline. Amy lase activity was significantly improved by different time ultraviolet irradiation. Enzyme activity was signifi cantly improved by 0.2% and 0.4% of colchicine, and 0.3% and 0.5% concentration treatment was significant ly higher than those in the control group. Effect of ultraviolet treatment was better than that of colchicine. A mong combination treatment, 10 groups of enzyme activity increased significantly. Enzyme activity presented two peak value in most of the treatments, which appeared in the second and the fifth day. Enzyme activity reached maximum in the first day or the second day of the treatment.

关 键 词：紫外线 秋水仙素 萌发率 淀粉酶 活性 陈年种子 

分 类 号：S511.041[农业科学—作物学]