The similarity between human embryonic stem cell-derived epithelial cells and ameloblast-lineage cells  

作　　者：Li-Wei Zheng Logan Linthicum Pamela K DenBesten Yan Zhang 

机构地区：[1]Department of Orofacial Sciences,University of California [2]Department of Biology,Humboldt State University

出　　处：《International Journal of Oral Science》2013年第1期1-6,共6页国际口腔科学杂志（英文版）

基　　金：supported by NIH/NIDCR grants R03 DE019507-02 to Yan Zhang,R21 D E0 18633 to Pamela K DenBesten and 2011SCU 11999-3/ NSFC81200760to Li-Wei Zheng

摘　　要：This study aimed to compare epithelial cells derived from human embryonic stem cells(hESCs) to human ameloblast-lineage cells (ALCs),as a way to determine their potential use as a cell source for ameloblast regeneration.Induced by various concentrations of bone morphogenetic protein 4(BMP4),retinoic acid(RA) and lithium chloride(LiCI) for 7 days,hESCs adopted cobble-stone epithelial phenotype(hESC-derived epithelial cells(ES-ECs)) and expressed cytokeratin 14.Compared with ALCs and oral epithelial cells(OE), ES-ECs expressed amelogenesis-associated genes similar to ALCs.ES-ECs were compared with human fetal skin epithelium,human fetal oral buccal mucosal epithelial cells and human ALCs for their expression pattern of cytokeratins as well.ALCs had relatively high expression levels of cytokeratin 76,which was also found to be upregulated in ES-ECs.Based on the present study,with the similarity of gene expression with ALCs,ES-ECs are a promising potential cell source for regeneration,which are not available in erupted human teeth for regeneration of enamel.This study aimed to compare epithelial cells derived from human embryonic stem cells （hESCs） to human ameloblast-lineage cells （ALCs）, as a way to determine their potential use as a cell source for ameloblast regeneration. Induced by various concentrations of bone morphogenetic protein 4 （BMP4）, retinoic acid （RA） and lithium chloride （LiCI） for 7 days, hESCs adopted cobble-stone epithelial phenotype （hESC-derived epithelial cells （ES-ECs）） and expressed cytokeratin 14. Compared with ALCs and oral epithelial cells （OE）, ES-ECs expressed amelogenesis-associated genes similar to ALCs. ES-ECs were compared with human fetal skin epithelium, human fetal oral buccal mucosal epithelial cells and human ALCs for their expression pattern of cytokeratins as well. ALCs had relatively high expression levels of cytokeratin 76, which ,vas also found to be upregulated in ES-ECs. Based on the present study, with the similarity of gene expression with ALCs, ES-ECs are a promising potential cell source for regeneration, which are not available in erupted human teeth for regeneration of enamel.

关 键 词：AMELOBLAST CYTOKERATIN dental epithelial cells human embryonic stem cells ODONTOGENESIS 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]