吉富罗非鱼群体遗传多样性的AFLP分析  

Genetic Diversity of GIFT Tilapia Populations by AFLP Analysis

作　　者：燕涛[1] 郭昱嵩[1] 王中铎[1] 刘丽[1] 刘楚吾[1] 

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,湛江524025

出　　处：《基因组学与应用生物学》2013年第2期207-211,共5页Genomics and Applied Biology

基　　金：广东省海洋渔业科技攻关与研发项目(A201008C03;A201101B02)资助

摘　　要：为了分析吉富罗非鱼的遗传多样性,为其育种提供理论依据,本实验利用5对引物组合(E-AGG/M-CTT,E-AGG/M-CTG,E-AAC/M-CAG,E-ACA/M-CTG,E-ACA/M-CAG)对广东省化州光辉养殖场有限公司新选育的吉富罗非鱼第16代群体(F16)进行遗传多样性的AFLP分析。27个个体共检出187个扩增位点,其中多态位点163个,平均每对引物扩增出32.6个位点,多态位点比例87.17%;平均Nei's基因多样性0.2886;平均Shannon's指数0.4339。结果说明实验群体存在较丰富的遗传多样性且保持有较高的遗传杂合度,具有进一步育种和开发的价值。The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia （GIFT） in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F 16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou （Guangdong, China） was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers based on 27 individuals, and among them 163 （87.17%） were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei＇s gene diversity was 0.288 6, average Shannon＇s information index was 0.433 9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.

关 键 词：吉富罗非鱼 扩增片段长度多态性(AFLP) 遗传多样性 多态位点比例 

分 类 号：Q959.4[生物学—动物学]