MTT法不适用于代谢水平改变的细胞活力检测  

Failure of MTT assay in application to cell with unstable metabolic level

作　　者：施文荣[1] 刘艳[1] 

机构地区：[1]福建中医药大学中西医结合学院,福州350108

出　　处：《生物学杂志》2013年第2期84-86,共3页Journal of Biology

基　　金：福建省自然科学基金项目(2011J01207)

摘　　要：利用MTT法和台盼蓝拒染计数法分别检测L02肝细胞经游离脂肪酸(FFA)处理的存活率,同时测定各组细胞琥珀酸脱氢酶(SDH)活性。结果 MTT法显示0.8 mmol/L FFA组细胞存活率较正常组显著增加(p<0.01),而台盼蓝拒染计数法却显示该组细胞存活率较正常组无明显改变(p>0.05),同时0.4 mmol/L、0.8 mmol/L FFA组细胞SDH活力显著增加(p<0.01)。研究表明,MTT法不适用于检测代谢水平改变的细胞生长、存活等指标。To investigate the feasibility of MTT for the detection of the survival rates of cells treated by free fatty acid（ FFA）, methyl thiazolyl tetrazolium（MTT） and trypan blue exclusion assay were performed respectively. MTT assay showed 0. 8 mmol/L FFA could significantly improve cell viability, but trypan blue exclusion assay showed the cell viability did not change obviously. The activity of mitochondrial succinate dehydrogenase rose significantly. The present data suggest MTT method should not be applied to ceils with un- stable metabolic level.

关 键 词：MTT法 台盼蓝拒染计数法 琥珀酸脱氢酶 

分 类 号：Q2-33[生物学—细胞生物学]