混合菌多样性分析及其浸出高砷金矿条件的优化  

Biodiversity Analysis for Bacterial Mixture and Optimization of Leaching High Arsenic-Containing Gold Ores

作　　者：袁学武[1] 谢学辉[1] 范凤霞[1] 朱文祥[1] 刘娜[1] 柳建设[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《矿冶工程》2013年第2期91-96,共6页Mining and Metallurgical Engineering

基　　金：国家自然科学基金项目(41073060);教育部博士点基金新教师类项目(20120075120014);上海市自然科学基金青年项目(12ZR1440400);上海市重点学科建设项目(B604);国家环境保护纺织工业污染防治工程技术中心项目

摘　　要：为了提高混合菌对高砷金矿的浸出,对混合菌进行了生物多样性分析,并对温度、矿浆浓度、初始pH值和接种量等工艺因素进行了优化,然后通过正交实验研究矿浆浓度、初始pH值和接种量对混合菌浸出高砷金矿中As和Fe的影响。限制性片段长度多态性分析(RFLP)结果发现,混合菌主要为Sulfobacillus属和Leptospirillum属。正交实验结果表明,浸出As的最佳条件为矿浆浓度10%,初始pH=1.5,接种量30%;浸出Fe的最佳条件为矿浆浓度5%,初始pH=1.5,接种量10%。在最佳条件下浸出20 d,As和Fe的浸出率分别达到97.12%和96.59%。Biodiversity of bacterial mixture was analyzed, and the influence of temperature, pulp density, initial pH and inoculation on the leaching of high arsenic-containing gold ores was investigated. Pulp density, initial pH and inocula- tion were studied by orthogonal design in order to obtain optimal leaching condition, including As and Fe from high arsenic-containing gold ores. RFLP analysis revealed that the bacterium fell into two putative divisions, which were Sulfobacillus and Leptospirillum. Orthogonal design results showed that optimal condition for leaching As was 10% pulp density, initial pH 1.5 and 30% inoculation, and optimal condition for leaching Fe was 5% pulp density, initial pH 1.5 and 10% inoculation. Under such optimal leaching condition, the leaching rate of As and Fe reached 97.12% and 96.59% after 20 d, respectively.

关 键 词：生物浸出 高砷金矿 混合菌 生物多样性分析 砷 铁 

分 类 号：TD925[矿业工程—选矿]