CTGF在OSAHS患者腭咽软组织中的表达及意义  

Expression of CTGF in pharyngeal tissue of patients with OSAHS and its significance

作　　者：吉雷[1] 张爱华[1] 詹俊杰[1] 

机构地区：[1]佳木斯大学附属第一医院耳鼻喉科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2013年第2期20-22,共3页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学研究生创新科研项目;编号:YJSCX2012-026JD

摘　　要：目的:通过检测阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)患者腭咽软组织中结缔组织生长因子(connective tissue growth factor,CTGF)的表达特征,探讨其与OSAHS患者病情程度和临床指标的相关性,从而研究其在OSAHS发生、发展过程中即:缺氧-CTGF升高-咽狭窄这一恶性循环中的作用。方法:实验对象为50例经多导睡眠图(PSG)监测确诊的OSAHS患者,以呼吸暂停低通气指数(AHI)为标准进行病情分度,其中轻中度30例,重度20例。对照组为25例慢性扁桃体炎患者。采用免疫组化SP法检测两组患者术中切除的腭咽软组织中CTGF的表达情况,同时光镜下观察分析两组标本的组织病理学特征。结果:CTGF的阳性表达率在轻中度OSAHS患者中为60%(18/30),在重度OSAHS患者中为90%(18/20),对照组CTGF染色阳性表达率24%(6/25);且轻中度组与对照组,重度组与对照组,以及轻中度组与重度组组间比较有显著性差异(P<0.05)。结论:本实验研究表明OSAHS患者腭咽软组织中CTGF的含量明显高于正常对照组。并且OSAHS患者中,重度组腭咽软组织中CTGF的含量高于轻中度组。CTGF可调控内皮细胞功能,还可促进微血管周细胞程序性死亡,导致血管渗透性增加,促进血管新生,在OSAHS患者咽腔狭窄,腭咽软组织松弛、血管扩张、间质水肿等一系列病理改变中可能发挥重要作用,其具体机制尚有待进一步研究。Objective： This article is to research the relative possible factors in obstructive sleep apnea hy- popnea syndrome （OSAHS） patients based on the detection of CTGF expression characteristics in pharyngeal tissue. Furthermore, it will investigate the relationship between the CTGF level in pharyngeal tissue with con- dition degree and clinical index in the patients with OSAHS. This research paper is to probe the effect of CTGF on the pathogenesis of OSAHS in the vicious cycle of hypoxia -- CTGF elevated -- pharynx stenosis. Meth- ods： Based on apnea hypopnea index （AHI） measured by polysomnography, 50 patients with OSAHS were di- vided into mild ＆ moderate OSAHS group（n= 30）,severe OSAHS group （n= 20）. The control group is the pa- tients with the chronic tonsillitis （n = 25）. The immunohistochemical S--P method techniques are amplified to study the expression of CTGF in the pharyngeal tissue of two group patients by light microscope observing the morphological changes of two group specimen. Results ： The positive rate of CTGF in mild ＆ moderate OSAHS group is 60% （18/30）, and it is 90% （18/20） in severe OSAHS group. The control group is 24% （6/25）. The finding shows that there are significant differences among the mild ＆ moderate OSAHS group and the con- trol group, the severe OSAS group and the control group, the mild and moderate OSAS group and the severe OSAS group （P 〈 0. 05）. Conclusion. The experimental research shows that the level of the CTGF content in the palatine pharyngeal tissue of OSAHS is obviously higher than that in the control group. And the level of CTGF content in the palatine pharyngeal tissue of severe OSAHS group is obviously higher than those in the mild and moderate OSAHS group. The results show that the CTGF plays an important role in the OSAHS, but the specific mechanism still needs further research.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 免疫组织化学 结缔组织生长因子 

分 类 号：R766[医药卫生—耳鼻咽喉科]