川芎嗪对酒精性股骨头缺血性坏死骨细胞凋亡影响的实验研究  

Experiment on the influence of ligustrazine on osteocyte apoptosis in alcohol-related necrosis of the femoral head

作　　者：蔡敏[1] 李宏宇[1] 来文兵[1] 杨勇[1] 

机构地区：[1]广西壮族自治区人民医院骨科,南宁530021

出　　处：《中国临床新医学》2013年第4期311-314,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西研究生教育创新计划资助项目(资助编号:2011105981002M188;指标编号:309366)

摘　　要：目的观察不同剂量川芎嗪对骨细胞凋亡率的影响和对骨组织的保护作用。方法将实验组48只雄性家兔采用灌胃法,给予烈性普通白酒(含体积分数50%乙醇)8ml/(kg·d),将实验组随机分成生理盐水对照组和3种不同剂量[8mg/(kg·d),4mg/(kg·d),2mg/(kg·d)]的盐酸川芎嗪干预组。另12只雄性家兔设为正常对照组不做任何处理。以上五组均用药观察至处死为止。于1、2、3、6个月分批处死,用TUNEL法检测各组间骨细胞凋亡情况。结果第3、6个月时大、中剂量川芎嗪组与生理盐水对照组相比较骨细胞凋亡数量逐渐减少,差异有统计学意义(P<0.05);第6个月时大、中剂量川芎嗪组与正常组相比较骨细胞凋亡数量已较为接近(P>0.05)。结论一定剂量范围、一定疗程的川芎嗪具有抑制骨细胞凋亡的作用。Objective To observe the effect of ligustrazine on osteocyte apoptosis rate in rabbits, and to ex- plore its protective effect. Methods Forty-eight New Zealand white rabbits were chosen and randomly divided into four groups. The experimental group was administered the strong distilled spirits containing 50% （v/v） of alcohol at a dosage of 8 ml/（ kg ~ d）. The experiment groups were divided into Saline control group, and high-, middle- and low- dosage ligustrazine [ 8,4 and 2 mg/（ kg ~ d） respectively] groups. Moreover 12 New Zealand white rabbits as a nor- mal control group received no treatment. The animals in five groups were sacrificed in batches 1, 2, 3 and 6 months after the procedures. The changes of histology in the Hepar and femoral head were observed. The osteocytes apoptosis was measured by means of TUNEL. Results At 3, 6 months, compared with the control group the number of osteo- cyte apoptosis in the high-, mlddle-dosage ligustrazine group gradually reduced. The apoptosis rate of osteocyte dif- fered from those in other groups （P 〈 0. 05 ）. At six months, the number of osteocyte apoptosis in high- middle-dos- age ligustrazine groups were close to the number of osteocyte apoptosis in the control group（P 〉 0.05）. Conclusion A certain range of dosage and course of ligustrazine has a protective effect on the apoptosis of osteocytes.

关 键 词：酒精性股骨头缺血性坏死 川芎嗪 凋亡 

分 类 号：R68[医药卫生—骨科学]