HPLC法测定血康颗粒中芍药苷的含量  

Determination of paeoniflorin in Xuekang Granules by HPLC

作　　者：程东方[1] 谢纪珍[2] 贾元印[3] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省药品审评认证中心,山东济南250014 [3]山东省中医药研究院,山东济南250014

出　　处：《药学研究》2013年第3期150-152,共3页Journal of Pharmaceutical Research

摘　　要：目的建立高效液相色谱法测定血康颗粒中芍药苷的含测方法。方法采用HPLC-ELSD法,用十八烷基硅烷键合硅胶为填充剂;以乙腈-0.1%磷酸溶液(14∶86)为流动相;检测波长231 nm,对样品中的芍药苷进行测定。结果通过方法学的系统考察和三批样品的含量测定,建立了制剂中芍药苷的高效液相色谱含测方法。其回归方程为Y=1 261 660.7X-578.7,r=0.999 8。结论建立的含量测定方法,具有灵敏度高、专属性强等特点,是控制本品内在质量的较理想方法。Objective To establish a method for the determination of paeoniflorin in Xuekang Granules by HPLC. Methods The content determination used HPLC - ELSD, chromatographic conditions were carried out as following ： column was packed with the octadecyl silane chemically bonded silica, flowing phase was composed with acetonitrile and 0. 1% phosphoric acid（14：86） and the detection wavelength was 23l nm. Results The HPLC method for determination of paeoni- florin in this preparation was established through methodology of systematic investigation and determination of three batch sample. The regression equation was Y = 1 261 660. 7X - 578.7, r = 0.999 8. Conclusion The method was sensitive, highly specific, and a the ideal way to control the quality of the products.

关 键 词：血康颗粒 高效液相色谱 芍药苷 含量测定 

分 类 号：R927.2[医药卫生—药学]