Sysmex UF-1000i尿有形成分分析仪对尿液真菌筛查的临床应用评价  

Evaluation on the clinical application of Sysmex UF-1000i urine formed element analyzer for screening funguria

作　　者：丁志祥[1] 蒋银芬[1] 周伊兰[1] 金文涛[1] 钱高潮[1] 

机构地区：[1]南京中医药大学附属常州市中医医院检验科,江苏常州213003

出　　处：《检验医学》2013年第4期290-292,共3页Laboratory Medicine

摘　　要：目的评价Sysmex UF-1000i尿有形成分分析仪(简称UF-1000i)在真菌尿筛查中的临床应用价值。方法分别测定UF-1000i检测白念珠菌的精密度、线性范围和携带污染率。选择UF-1000i提示类酵母细胞(YLC)阳性样本200例,阴性样本400例,同时做显微镜检查。结果 UF-1000i检测白念珠菌的精密度为3.83%～4.71%,线性范围为10.0～5 873.1个/μL,线性相关系数(r)为0.999,携带污染率为0.12%。以显微镜检查为金标准,受试者工作特征(ROC)曲线结果显示UF-1000i检测真菌YLC的最佳临界值为18.05个/μL,其检测真菌的敏感性、特异性、准确度、阳性预测值和阴性预测值分别为97.0%、85.6%、87.5%、57.4%和99.3%。结论 UF-1000i可快速提供真菌孢子定量检测结果,辅助诊断尿路真菌感染。Objective To evaluate the clinical application significance of Sysmex UF-1000i urine formed element analyzer （UF-1000i analyzer） for screening funguria. Methods The precision, linear range and carryover rate of Candida albicans were detected by UF-IO00i analyzer. A total of 200 urine samples which UF-1000i analyzer showed like yeast eell（YLC） positive and 400 YLC negative urine samples were detected by manual microscopy. Results For UF-1000i analyzer detecting Candida albicans, the precision was 3. 83%-4. 71%, the linear range was 10. 0-5 873.1 cells/μL,the correlation coefficient was 0. 999, and the carryover rate was 0.12%. Manual microscopy was as the gold standard, and the result of receiver operating characteristic （ ROC ） curve showed the best cut-off value for UF-1000i analyzer detecting YLC was 18.05 cells/μL. The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of UF-1000i analyzer in the diagnosis of funguria were 97.0% ,85.6% ,87.5% ,57.4% and 99.3%, respectively. Conclusions UF-1000i analyzer can provide quantitative results of fungal spores. It aids to diagnose urinary tract fungal infection.

关 键 词：真菌尿 SYSMEX UF-1000i尿有形成分分析仪 显微镜检查 

分 类 号：R446.11[医药卫生—诊断学]