甲状腺球蛋白抗体竞争TRFIA技术的建立及临床应用  

Establishment of TRFIA for anti-thyroglobulin antibody and its clinical application

作　　者：朱岚[1] 黄飚[1] 张珏[1] 陈永伟[1] 李跃松[1] 

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室,江苏无锡214063

出　　处：《检验医学》2013年第4期305-307,共3页Laboratory Medicine

摘　　要：目的建立甲状腺球蛋白抗体(TgAb)时间分辨荧光免疫分析(TRFIA)竞争检测法。方法用甲状腺球蛋白抗原包被96孔板,用铕(Eu3+)标记羊抗鼠IgG做标记物,TRFIA检测人血清中的TgAb。结果 TgAb-TRFIA的敏感性为1.0 IU/mL;批内变异系数(CV)为3.3%～4.1%,批间CV为3.7%～4.7%;平均回收率为97.9%;热稳定性好;与电化学发光分析技术(ECLIA)比对,相关系数达0.881 3;男女TgAb测定值差异无统计学意义;TgAb的正常值范围为≤110 IU/mL。结论建立的TgAb-TRFIA是一个高敏感和可靠的检测方法,有助于甲状腺疾病的临床诊断。Objective To establish a time-resolved fluoroimmunoassay （TRFIA）for anti-thyroglobulin antibody （TgAb）. Methods The thyroglobulin antigen was coated on the 96 well plates. A goat anti-mouse IgG-europium （ Eu^3＋ ） was used to the detection. Serum TgAb level was detected by TRFIA. Results The sensitivity was 1.0 IU/ mL. The within-run coefficient of variation （CV） was 3.3%-4. 1%. The between-run CV was 3.7%-4.7%. The average reeovery was 97.9%. The kit was stable. The correlation coefficient with TgAb by electrochemiluminescence immunoassay （ECLIA） was 0. 881 3. The TgAb levels between males and females had no statistical significance. The normal range of TgAb was ≤ 110 IU/mL. Conclusions The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay, which would be useful for the clinical diagnosis of thyroid diseases.

关 键 词：时间分辨荧光免疫分析 甲状腺球蛋白抗体 甲状腺疾病 

分 类 号：R446.61[医药卫生—诊断学]