洋金花对帕金森病大鼠脑纹状体组织SOD、GSH-Px影响的研究  

Experimental Study on the Effects of Yangjinhua on Striatum Tissue SOD,GSH-Px of Model Rats with Parkinson's Disease

作　　者：金泽[1] 王玉琳[1] 姜珊珊[1] 侯凤祥[1] 王春英[1] 陈静[1] 王琳晶[1] 包大鹏[1] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001

出　　处：《中医药信息》2013年第2期87-90,共4页Information on Traditional Chinese Medicine

基　　金：博士后研究人员落户黑龙江科研启动资助金(Ibh-910004)

摘　　要：目的:采用单味药洋金花细粉对PD模型大鼠进行治疗,并探讨其作用机制,从而为中药治疗帕金森病提供一条新的思路。方法:采用脑立体定位注射6-OHDA建立大鼠PD模型,阿朴吗啡(Apo-morphine)诱导进行旋转行为观察,将模型成功大鼠随机分为洋金花高、中、低剂量组、美多芭组及模型对照组,同时设假手术组作为对照。观察其对超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)变化的影响。结果:给药3周后,各治疗组纹状体内MDA含量均有明显降低(P<0.01),以美多芭组、洋金花高、中剂量组降低显著,三组之间比较无显著性差别(P>0.05);各治疗组纹状体内SOD含量均有明显增加(P<0.05),以美多芭组、洋金花高剂量组增加显著,两组之间比较无显著性差别(P>0.05);各治疗组纹状体内GSH、GSH-Px均有明显增加(P<0.05),其中以洋金花中剂量组增加最明显。结论:洋金花可以降低PD模型大鼠纹状体MDA含量,提高GSH、GSH-Px、SOD的含量,可能是通过减轻脂质过氧化反应,增强抗氧化防御机制,减少氧化应激,进而保护神经元,起到治疗PD的作用。Objective ： Use single herbs Yangjinhua fine powder to treat rat model with PD to explore some of its mechanism, so as to provide a new way of Chinese medicine to treat Parkinson＇s disease. Method： Use ster- eotaxic injection of 6 - OHDA to establish rat models with PD, apomorphine induced rotational behavior obser- vation, the model rats were randomly divided into Yangjinhua high , medium and low -dose groups, the Madopar group and the model control group, at the same time set up the sham group as comparison. Observe the impact of changes of superoxide dismutase （ SOD）, malonic aldehyde （MDA） and glutathione （ GSH）, glutathione peroxidase （GSH -Px）. Results： After administration 3W, the striatum of MDA content of each treatment group was significantly lower （ P 〈 0.01 ）, to the Madopar group, the Yangjinhua high and medium dose groups decreased significantly, among the three groups there was no significant difference （P 〉 0.05 ） ; the SOD content of each treatment group Striatum was significantly increased （ P 〈 0. 05 ）. to the Madonar group, the Yangjinhua high dose group increased significantly, between the two groups there was no significant difference （ P 〉 O. 05 ） ; Each treatment group striatum, GSH, GSH - Px were significantly increased （ P〈 0. 05 ）, with the most obvious increase in the Yangjinhua medium dose group. Conclusion ： Yangjinhua can re- duce the content of PD model rat striatum MDA, improve the content of GSH, GSH - Px, SOD, the mecha- nism may be by reducing lipid peroxidation, enhancing the antioxidant defense mechanism, reducing the oxi- dative stress, thereby to protect the neurons, and plays a role in the treatment of PD.

关 键 词：洋金花 帕金森病 实验研究 自由基 

分 类 号：R285.5[医药卫生—中药学]