Electron microscopy:essentials for viral structure,morphogenesis and rapid diagnosis  

作　　者：ZHANG Ying HUNG Tao SONG JingDong HE JinSheng 

机构地区：[1]College of Life Sciences and Bioengineering,Electron Microscopy Laboratory,School of Science,Beijing Jiaotong University [2]Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention

出　　处：《Science China(Life Sciences)》2013年第5期421-430,共10页中国科学（生命科学英文版）

基　　金：supported by the Major Scientific and Technological Special Project of China(2009ZX10004-216);the National Natural Science Foundation of China(81100809,81271417);Fundamental Research Funds for the Central University of China(2011JBM124)

摘　　要：Electron microscopy(EM) should be used in the front line for detection of agents in emergencies and bioterrorism,on accounts of its speed and accuracy.However,the number of EM diagnostic laboratories has decreased considerably and an increasing number of people encounter difficulties with EM results.Therefore,the research on viral structure and morphology has become important in EM diagnostic practice.EM has several technological advantages,and should be a fundamental tool in clinical diagnosis of viruses,particularly when agents are unknown or unsuspected.In this article,we review the historical contribution of EM to virology,and its use in virus differentiation,localization of specific virus antigens,virus-cell interaction,and viral morphogenesis.It is essential that EM investigations are based on clinical and comprehensive pathogenesis data from light or confocal microscopy.Furthermore,avoidance of artifacts or false results is necessary to exploit fully the advantages while minimizing its limitations.Electron microscopy （EM） should be used in the front line for detection of agents in emergencies and bioterrorism, on accounts of its speed and accuracy. However, the number of EM diagnostic laboratories has decreased considerably and an increasing number of people encounter difficulties with EM results. Therefore, the research on viral structure and morphology has be- come important in EM diagnostic practice. EM has several technological advantages, and should be a fundamental tool in clinical diagnosis of viruses, particularly when agents are unknown or unsuspected. In this article, we review the historical contribution of EM to virology, and its use in virus differentiation, localization of specific virus antigens, virus-cell interaction, and viral morphogenesis. It is essential that EM investigations are based on clinical and comprehensive pathogenesis data from light or confocal microscopy. Furthermore, avoidance of artifacts or false results is necessary to exploit fully the advantages while minimizing its limitations.

关 键 词：electron microscopy viral structure viral morphology viral diagnosis 

分 类 号：Q939.4[生物学—微生物学]