蛋白酶体亚基LMP-2、Survivin、Caspase-3在稽留流产绒毛及蜕膜组织中的表达  

Express of proteasome subunit LMP-2,Survivin and Caspase-3 in missed abortion patients

作　　者：潘铁梅[1,2] 宋曙 陆秀萍[4] 曹森杨 陈亦成 陈永珍[2] 

机构地区：[1]江苏省盐城市妇幼保健院生育技术科,盐城224002 [2]苏州大学医学部基础医学与生物科学学院解剖与组织胚胎学系 [3]盐城市第一人民医院病理科 [4]盐城市妇幼保健院妇科 [5]盐城市妇幼保健院病理科

出　　处：《南通大学学报（医学版）》2013年第2期94-97,F0003,共5页Journal of Nantong University(Medical sciences)

基　　金：苏州大学横向基金资助项目(P113400411);盐城市医学科技发展计划资助项目(YK2012026)

摘　　要：目的:检测稽留流产绒毛及蜕膜组织中蛋白酶体亚基低分子质量多肽-2(proteasome subunit low molecu-lar weight polypeptide 2,LMP-2)、半胱氨酸蛋白酶-3(cysteine-containing aspartate specific protease 3,Caspase-3)、生存素(Survivin)的表达,旨在探讨稽留流产的发病机制。方法:采用免疫组织化学染色方法检测30例稽留流产(实验组)患者绒毛及蜕膜组织中LMP-2、Caspase-3、Survivin的表达,并以30例正常早孕人工流产绒毛及蜕膜组织为对照组。结果:稽留流产组绒毛及蜕膜中LMP-2、Survivin的表达均低于正常早孕组,Caspase-3的表达则高于正常早孕组,差异均有统计学意义(P<0.05)。实验组的绒毛及蜕膜Caspase-3与LMP-2及Survivin的表达均呈负相关,LMP-2与Survivin的表达呈正相关(P<0.05)。结论:LMP-2、Survivin及Caspase-3的异常表达在稽留流产的发生中起重要作用。Objective： To study the relationship of proteasome subunit proteasome subunit low molecular weight polypeptide 2（LMP-2）, Survivin and cysteine-containing aspartate specific protease 3（Caspase-3） with missed abortion. Methods： Thirty missed abortion patients were assigned as study group, and thirty with normal pregnancy as controls. Inmunohistochemical method was used to determine the expnession of LMP-2, Survivin and Caspase-3. Results： Compared with that of the controls, the expression of LMP-2 and Survivin in the study group were significantly lower,while the expressions of Caspase-3 were higher（P〈0.05）. In the study group, the Caspase-3 expression was negatively correlated with the expression of LMP-2 and Survivin respectively, and the expressions of LMP-2 was found to be positively correlated with Survivin （P〈0.05）. Conclusion： The Abnormal expressions of LMP-2, Survivin and Caspase-3 play important role in missed abortion.

关 键 词：稽留流产 凋亡 蛋白酶体亚基低分子质量多肽-2 半胱氨酸蛋白酶-3 生存素 

分 类 号：R714.21[医药卫生—妇产科学]