紫外辐照联合多聚甲醛熏蒸法制备真菌扫描电镜样品的初探  

A study of preparation of pathogenic fungi samples for SEM observation by UV irradiation combined with paraformaldehyde steam fumigation

作　　者：钱闻博[1] 张天一[2] 沈永年[3] 朱昌来[2] 

机构地区：[1]南通大学医学院 [2]南通大学神经再生重点实验室,江苏226001 [3]中国协和医科大学皮肤病研究所,南京210042

出　　处：《交通医学》2013年第1期24-26,共3页Medical Journal of Communications

摘　　要：目的:探讨简易的扫描电镜样品制备方法,观察病原丝状真菌菌丝、孢子表面形貌的超微结构特征。方法:采用玻片小培养法制备病原丝状真菌的菌株,应用紫外辐照联合多聚甲醛蒸汽熏蒸法对样品进行消毒、固定,并经金属喷镀后在扫描电镜下观察。结果:扫描电镜下,该方法能保留所有菌株的菌丝及孢子原有的排列方式,并且能在扫描电镜下表现出良好的超微结构特征。结论:紫外辐照-多聚甲醛熏蒸法处理病原丝状真菌样品,能简单、快捷的用于扫描电镜观察,并可为相关真菌的分类提供形态学依据。Objective：To explore a simple method of preparation of pathogenic fungi samples for observation of mor- phology by scanning electron microscopy （SEM）. Methods： Strains of filamentous fungus were obtained by slide culture method, and then the samples were prepared by UV irradiation combined with paraformaldehyde steam fumigation and ob- served under SEM after metal spraying. Results： The fungal conidia and hyphae were well preserved and clearly showed the morphology of isolates under SEM. Conclusion： UV irradiation combined with paraformaldehyde steam fumigation is a simple and rapid method for SEM observation of prepared fungal samples, which may contribute to providing morphological evidence for classification of pathogenic fungi.

关 键 词：病原丝状真菌 紫外辐照法 多聚甲醛熏蒸法 扫描式电镜检查 

分 类 号：Q939[生物学—微生物学]